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Multipotent Genetic Suppression of Retrotransposon-Induced Mutations by Nxf1 through Fine-Tuning of Alternative Splicing

Figure 7

Nxf1CAST does not suppress ETn-induced mutations in A/J.

(A) Genomic organization of five sense and three antisense-oriented ETn insertions monitored in F2 mice from A/J x B6–Nxf1CAST. Insertions are not to scale. (B) Quantitative PCR (SYBR green) on brain and/or muscle cDNA shows reduced expression of sense-oriented ETn alleles, but no significant differences between Nxf1 genotypes. m, insertion allele at each indicated locus. (C) Quantitative PCR shows modest (Atp9a) or no difference (Art3, Mark3) in expression of antisense-oriented ETn insertions, with no difference attributable to Nxf1. Error bars indicate standard deviation.

Figure 7

doi: https://doi.org/10.1371/journal.pgen.1000484.g007