Genetic and Computational Identification of a Conserved Bacterial Metabolic Module
Figure 1
Biochemical pathway of myo-inositol degradation in C. crescentus.
Functional assignments of the catabolic genes are based on biochemical work in B. subtilis [14]. All of the genes for this catabolic pathway are present in the C. crescentus myo-inositol catabolic locus diagrammed in Figure 2, except the gene iolJ. Protein CC3250, annotated as a fructose bisphosphate aldolase, has a high similarity to IolJ of B. subtilis (BLAST score<e−40). This gene is upregulated 1.9-fold in myo-inositol relative to glucose. We propose it is the most likely candidate to carry out the enzymatic function of IolJ in C. crescentus.