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Shaping Skeletal Growth by Modular Regulatory Elements in the Bmp5 Gene

Figure 1

Multiple enhancers control Bmp5 rib expression.

A. Schematic of the Bmp5 locus with positions of previously described regulatory alleles (Bmp5se38DSD, Bmp5se4CHLd), BACs, and the Ex4r and Phage 7 genomic clones used in transgenic analysis. B. Schematic of coronal sections through the torso and a rib (boxed) showing the orientation used in all rib cross-sections. C, D. Bmp5 in situs on rib cryosections from E13.5 wild-type (C) and Bmp5se38DSD mutant (D) embryos. Note the reduced expression in the anterior, medial and posterior perichondrial domains of the Bmp5se38DSD rib, with continued expression in the lateral domain (asterisk). E. Lateral view of BAC199-lacZ transgenic embryo. Red and grey bars represent planes of dorsal (red) and ventral (grey) rib sections. F, G. Set of rib cryosections taken from dorsal (F) and ventral (G) planes from a BAC199 embryo. H. Lateral view of BAC178-lacZ transgenic embryo. I, J. Set of rib cryosections taken from dorsal (I) and ventral (J) planes from a BAC178 embryo. Overall Bmp5 expression in rib perichondrium (C) is a mosaic pattern controlled by different regulatory regions for different spatial domains around the rib.

Figure 1

doi: https://doi.org/10.1371/journal.pgen.1000308.g001