Identification and Dissection of a Complex DNA Repair Sensitivity Phenotype in Baker's Yeast
Figure 1
BY/RM hybrid progeny show altered sensitivity to the DNA damaging agent, 4-NQO.
Phenotype testing of BY-RM hybrid segregants. Saturated cultures of BY, RM, rad52Δ (4-NQO control), and representative progeny of the BY×RM cross (segregants 47–54) were diluted in water and spotted in 10-fold serial dilution (undiluted to 10−5) onto YPD with or without 0.25 µg/ml 4-NQO. Plates were photographed after 2-day incubation at 30 C. Strains were scored as very sensitive (- -), sensitive (-), slightly sensitive (±), wild-type resistance (+) and increased resistance (+ +). The scoring method was also used in Table 1 and Figures 3, 4, 6, and 7.