Metabolic Actions of Estrogen Receptor Beta (ERβ) are Mediated by a Negative Cross-Talk with PPARγ
Figure 1
ERβ inhibits PPARγ activity in a ligand-independent manner.
A) 3T3-L1 preadipocytes were transfected with the indicated plasmids together with pGal4-hPPARγDEF, pG5TkGL3 and renilla, followed by treatment with 10 µM pioglitazone, 100 nM E2, or in combination as indicated. # p<0.05 vs. pSG5+veh; * p<0.05 vs. pSG5+Pio, ns: not significant vs. pSG5+Pio, nsa: not significant vs. ERβ+Pio. B) 3T3-L1 preadipocytes were transfected with ERβ (as indicated), and protein level of ERβ was analysed by Western blot. C) 3T3-L1 preadipocytes were transfected with the indicated plasmids together with pERE-TkGL3, and cells were treated with 100 nM E2, as indicated. * p<0.05 vs. pSG5+E2; ns: not significant vs. pSG5+veh.