Network Discovery Pipeline Elucidates Conserved Time-of-Day–Specific cis-Regulatory Modules
Figure 8
The PBX/TBX/SBX cis-Regulatory Module Controls Condition Specific Diurnal and Circadian Transcription
(A) z-Score profiles of words that make up the PBX (ATGGGCC) under LDHH (black) and LLHC (red).
(B) 3xPBX::LUC cycles under LDHH. Four to six seedlings from several independent T2 lines were analyzed and averaged. Results from three independent experiments are shown (Experiment number 185 n = 10, 186 n = 4, and 188 n = 3).
(C) PBX cycles under all diurnal and circadian conditions tested. Heat map displaying the phase of the 3xPBX::LUC lines from two independent experiments under four different experimental conditions, LDHH (black bar, dark), LLHC (blue bar, cold), LL_LDHH (grey bar, subjective night), and LL_LLHC (light blue bar, subjective night). The predicted phase for LDHH and LLHC is displayed below heat maps (red square). Heat map from high relative expression to low (purple, green, yellow, red, and blue).
(D) EE (black) and GATA (red) z-score profiles have distinct phases of overrepresentation, and share the GATA core but differ at flanking sequence.
(E) z-Score profile of the consensus EE shifts phase between LDHC (black) and LLHC (red).
(F) z-Score profile of the consensus TBX shifts phase between LDHH_ST (black) and LLHC (red).