Dominant-Negative CK2α Induces Potent Effects on Circadian Rhythmicity
Figure 5
CK2αTik Circadian Overexpression Alters PER Protein Levels and Mobility
(A) Representative PER western blots demonstrating differences in PER protein amount and mobility with UASTik overexpression during constant conditions. (a) y w, (b) timGal4/+; UASTikR/+, (c) Tik/+, (d) timGal4/+; UASTikT1/+, and (e) timGal4; UASTikT1.
(B) Quantification of PER protein levels indicate that decreasing CK2α function results in elevated levels and delayed decline of PER. +/+: y w, timTik: timGal4/+; UASTikT1/+; timTik2x: timGal4; UASTikT1. CT: circadian time. Quantification done from three independent experiments.