Involvement of Synaptonemal Complex Proteins in Sex Chromosome Segregation during Marsupial Male Meiosis
Figure 7
Immunolocalization of SCP3 (Red) and SCP1 (Green) and Counterstaining with DAPI (Blue) in a Spermatocyte at Diplotene
Two focal planes are superimposed in each picture.
(A) SCP3 lines representing the autosomal SC appear discontinuous (arrowheads). The sex chromosomes (X, Y) (enlarged in D–F) appear associated and the DP is detectable.
(B) SCP1 follows a similar pattern to that of SCP3 on the autosomes, appearing as fragmented lines.
(C) Merge of SCP3 and SCP1 signals, which co-localize in the autosomes but not in the sex chromosomes.
(D) Enlargement of the sex chromosomes. SCP3 appears along the AEs of the sex chromosomes and also in the DP.
(E) SCP1 does not appear in the AEs of sex chromosomes, but is present in the DP (arrow).
(F) Merge of the signals shown in (D) and (E). Note that the distribution of SCP1 in the DP is more restricted at this stage than that of SCP3.
(G) Schematic representation of the sex chromosomes.