M-Track: A New Software for Automated Detection of Grooming Trajectories in Mice
Fig 1
Sensitivity of grooming to mild and strong stressor exposure.
(A) Relationship between the mean duration of grooming episodes in mice tested before and immediately after being handled to color their forepaws (before: 0.32±0.05 min, after: 0.69±0.07 min (n = 47), ***p = 5.9e-6). (B) Relationship between the mean frequency of grooming episodes in mice tested before and immediately after handling/coloring the forepaws (before: 0.010±0.001 Hz, after: 0.012±0.001 Hz (n = 47), p = 0.051). (C) Relative change in the duration and frequency of grooming episodes in mice tested immediately after being handled to color their forepaws (duration: 2.68±0.22, frequency: 1.61±0.23 (n = 47)). (D) Relationship between the mean duration of grooming episodes in mice tested before and 20 min after being handled to color their forepaws (before: 0.25±0.02 min, after 0.33±0.06 min (n = 16), p = 0.098). (E) Relationship between the mean frequency of grooming episodes in mice tested before and 20 min after handling/coloring the forepaws (before: 0.015±0.002 Hz, after: 0.012±0.002 Hz (n = 16), p = 0.582). (F) Relative change in the duration and frequency of grooming episodes in mice monitored 20 min after handling the mice to color their forepaws (duration: 1.47±0.26, frequency: 1.14±0.23 (n = 16)). (G) Relationship between the mean duration of grooming episodes in mice tested before and after 5 min exposure to bright light (before: 0.20±0.03 min, after: 0.40±0.08 min (n = 24), *p = 0.014). (H) Relationship between the mean frequency of grooming episodes in mice tested before and after 5 min bright light exposure: 0.012±0.002 Hz, 0.011±.001 Hz (n = 24), p = 0.636). (I) Relative change in the duration and frequency of grooming episodes in mice exposed to bright light (duration: 2.31±0.54, frequency: 1.34±0.21 (n = 24)). (J) Relationship between the mean duration of grooming episodes in mice tested before and after restraint stress (before: 0.18±0.02 min, after: 0.49±0.07 min (n = 15), ***p = 8.9e-4). (K) Relationship between the mean frequency of grooming episodes in mice tested before and after restraint stress: 0.008±0.001 Hz, 0.013±0.002 Hz (n = 15), *p = 0.018). (L) Relative change in the duration and frequency of grooming episodes induced by 30 min restraint stress (duration: 3.29±0.57, frequency: 1.90±0.33 (n = 15)).