MIiSR: Molecular Interactions in Super-Resolution Imaging Enables the Analysis of Protein Interactions, Dynamics and Formation of Multi-protein Structures
Fig 5
Quantification of the lysosomal marker LAMP1 in APP vesicles using the DBSCAN algorithm.
(A) GSDM image analyzed in panels B-D. All analyses are performed on the region within the insert. (B) Identification of clusters using the DBSCAN algorithm using two different values for minimum cluster size (k) and neighbourhood size (ε), with individual clusters identified by color. (C) Top: Regions of Interest (ROIs) defined by the edge points of APP clusters identified using DBSCAN values of k = 10 and ε = 100; individual clusters are identified by color. Bottom: APP-defined ROIs overlaid on LAMP1 staining. (D) Quantification of LAMP1 density inside of APP clusters versus APP cluster sizes. Images are representative of, and graph quantifies, images from 3 experiments with a minimum of 4 images per experiment. Data is presented as mean ± SEM.