Combinatorial Pooling Enables Selective Sequencing of the Barley Gene Space
Figure 1
(A) obtain a BAC library for the target organism; (B) select gene-enriched BACs from the library (optional); (C) fingerprint BACs and build a physical map; (D) select a minimum tiling path (MTP) from the physical map; (E) pool the MTP BACs according to the shifted transversal design; (F) sequence the DNA in each pool, trim/clean sequenced reads; (G) assign reads to BACs (deconvolution); (H) assemble reads BAC-by-BAC using a short-read assembler.