Stochastic De-repression of Rhodopsins in Single Photoreceptors of the Fly Retina
Figure 2
Rh3, Rh5 and Rh6 are de-repressed in dve mutants.
(A) Three dimensional rendering of a representative confocal stack of a retina dissected at the 2 week developmental time point. Phalloidin, which stains actin, was used to visualize the rhabdomeres (green). This retina is co-stained for Rh5 (red) and Rh6 (blue). The inset shown is a zoomed-in view of the center of the retina. (B & C) Retinae were co-stained for two Rhodopsins, and representative ommatidia extracted automatically from the image stacks are shown for retinae co-stained for either Rh3–Rh6 or Rh5–Rh6 (panels B-i & C-i). Panels B-ii & C-ii show a cross section of the ommatidium in the phalloidin channel, indicating the automatically identified PR cells (R1–R6; R7/R8). Rh3 levels exhibit de-repression in outer PR cells (B-iii). Rh6 levels exhibit de-repression in outer PR cells (panels B-iv & C-iv). Rh5 levels exhibit de-repression in outer PR cells (C-iii). Scale bar is 1.5 µm (panels B) and 1.0 µm (panels C).