Modeling Mitochondrial Bioenergetics with Integrated Volume Dynamics
Figure 7
Model predictions (line) of the steady state extra-mitochondrial α-ketoglutarate concentrations during state 2 respiration with various extra-mitochondrial malate concentrations (symbols) is compared to the experimentally reported values outlined in LaNoue et al. [9].
Mitochondria were incubated in the assay buffer identified in the Methods section under the LaNoue data set description. Extra-mitochondrial malate was incrementally increased from 0 to 5 mM in the presence of 2 mM pyruvate. The model was simulated at sufficient times to achieve α-ketoglutarate steady state concentrations.