A Bipolar Clamp Mechanism for Activation of Jak-Family Protein Tyrosine Kinases
Figure 6
Potencies of SH2B-β domain mutants as dominant negatives antagonizing wild-type SH2-Bβ function.
Jak2 phosphorylation was calculated using the Extended Cellular Model as in Figure 5A, with the same parameter values and PTot = 1 µM. To this model, we added one of the following SH2-Bβ constructs: SH2 only (A), DD only (B), PH-SH2 (C), and DD-PH (D). As indicated, the value of the overall inhibitor concentration was either 0 (no inhibition; same as Figure 5A), 100 nM, 1 µM, or 10 µM.