FMRP activity and control of Csw/SHP2 translation regulate MAPK-dependent synaptic transmission
Fig 4
Activity-dependent synaptic plasticity repressed in csw/PTPN11 mutants.
Synaptic plasticity during and following a short-term stimulation train to measure facilitation, augmentation, and PTP. (A) Stimulation paradigm: 1 minute at 0.2 Hz (0.2 mM Ca2+), followed by 1 minute at 10 Hz, and then a return to 0.2 Hz. (B) Sample EJC traces at indicated time points during and following the 10 Hz train for control (w1118, top), GoF PTPN11N308D (elav-Gal4>PTPN11N308D; middle), and csw null (csw5, bottom). (C) Quantification of EJC amplitude during the 10-Hz train normalized to basal EJC amplitude for each genotype. The nonlinear regression exponential for each pair tested using extra sum-of-squares F test. (D-F) Quantification of facilitation (1 second, D) and augmentation (30 seconds, E) during the 10-Hz train, and PTP (10 seconds following train, F) normalized to the basal EJC amplitude for each genotype using Mann–Whitney/two-sided t tests. Scatter plots show all data points and mean ± SEM. N = number of NMJs. Significance: p < 0.05 (*), p < 0.001 (**), and p < 0.0001 (****). The data underlying this figure can be found in S1 Data. csw, corkscrew; EJC, excitatory junction current; GoF, gain-of-function; NMJ, neuromuscular junction; PTP, post-tetanic potentiation; PTPN11, protein tyrosine phosphatase non-receptor type 11.