Reestablishment of spermatogenesis after more than 20 years of cryopreservation of rat spermatogonial stem cells reveals an important impact in differentiation capacity
Fig 3
Rat germ cell identity assignments.
(A) Heatmaps showing selected gene expression of aggregate normalized counts per cell type in unselected and EpCAM+ cell types. Cell type assignments were applied to unsupervised clusters generated in Fig 2 based on expression of marker genes corresponding to known cell types in Fig 1A. Underlying data shown in S4 Data. (B) Ingenuity pathway analysis of selected pathways by cell type in germ cells. Shown are EpCAM-derived clusters for early germ cell states (black text) and unselected for postmeiotic cells (purple text). (C) Unselected germ cell cluster assignments. (D) EpCAM+ germ cell cluster assignments. All underlying data deposited in NCBI GEO repository (GSE182438). SSC, spermatogonial stem cell; UMAP, uniform manifold approximation and projection.