Endomitosis controls tissue-specific gene expression during development
Fig 7
Transient activation of vitellogenin expression rescues defects of animals with mononucleated intestines.
(A) F2 brood sizes of Pges-1::TIR1; AID::knl-1 animals containing deletions in either vit-5, vit-6, or both. F2 brood sizes were measured from the progeny of animals with either mononucleated (+, n = 43 to 44) or binucleated (ct, n = 41 to 44) intestines that had been laid during the first day of adulthood (maternal age 48–72), in 2 replicate experiments. Animals without vit-5 or vit-6 deletions (wild type) were taken along as controls. (B) Normalized total Pvit-2::NLSGFP fluorescence intensity per animal, for animals grown under auxin (+) or control (ct) conditions and with (+, n = 17 and 29) or without (−, n = 82 and 89) intestinal overexpression of transcription factors ceh-60 and unc-62, measured at young adulthood in 5 replicate experiments. Scatter plot with line and error bars indicating mean and SEM. (C) Normalized total fluorescence intensities of BODIPY lipid staining of early embryos isolated from young adult (72 hours) Pges-1::TIR1; AID::knl-1 animals with or without intestinal overexpression of CEH-60 and UNC-62, grown under control (ct, n = 76 and 64) or auxin (+, n = 67 and 64) conditions in 3 replicate experiments. Boxplots indicate the median and 25th to 75th percentile, error bars indicate min to max values, and individual values are shown as dots. (D) F2 brood sizes of Pges-1::TIR1; AID::knl-1 animals with or without intestinal overexpression of CEH-60 and UNC-62. F2 brood sizes were measured from the progeny of animals with either mononucleated (+, n = 42 and 42) or binucleated (ct, n = 40 and 41) intestines that had been laid during the first day of adulthood, in 2 replicate experiments. P values were calculated by Mann–Whitney test. NS, not significant (P > 0.05). Underlying data can be found in S1 Data. AID, auxin-inducible degron.