Fast near-whole–brain imaging in adult Drosophila during responses to stimuli and behavior
Fig 4
Functional components match anatomical structures.
A) Components automatically sorted by region and projected along the z-axis. Note that the sorting (the component’s maps are averaged in anatomical regions of interest) could be inaccurate in the case of maps containing small parts of large regions and noise in very small regions (i.e., the bulb or gall). Data can be found on CNCRS.org (http://dx.doi.org/10.6080/K01J97ZN). B) Comparison between functional and anatomical maps. Left: functional maps from a pan-neuronal (GMR57C10-Gal4 and Cha-Gal4) GCaMP6f experiment. Right: corresponding anatomical structures. Maps were constructed using the Virtual Fly Brain database. Three bottom images show neurons from major neuron types (three different Kenyon cell types, antennal lobe projection neuron, and transmedullar neurons) matching the functional maps. The brain for the functional data is tilted 19 degrees along the lateral axis compared to the template presented on the right. Note that functional maps in the fan-shaped body are the same scale as functional maps obtained with microscopy techniques with higher resolution [17]. See also S13–S17 Figs for consideration regarding the associated time series. Cha-Gal4, choline acetyltransferase-Gal4; CNCRS, Collaborative Research in Computational Neuroscience.