Alteration of protein function by a silent polymorphism linked to tRNA abundance
Fig 3
T2562G-CFTR exhibits 2 types of channel openings.
(A) Representative single-channel recordings of CFTR variants in excised inside-out membrane patches from CHO cells. For wild-type and T2562C-CFTR, the membrane patches contained 1 channel; for T2562G-CFTR, it contained 2 channels—one with small conductance (sc) and the other with wild-type–like (wtl) openings. Dashed lines indicate the closed channel state and downward deflections correspond to channel openings. For T2562G-CFTR, the indicated 2-s portion is shown on an expanded time scale after filtering digitally. (B–E) Single-channel current amplitude (i), open probability (Po), mean burst duration (MBD), and interburst interval (IBI) of CFTR variants. Data are means ± SEM (n = 5–9); * P < 0.05 versus wild-type CFTR; ND, not determined. The underlying data of panels B–E can be found in S1 Data.