Epithelial Tumors Originate in Tumor Hotspots, a Tissue-Intrinsic Microenvironment
Fig 2
Endogenous JAK/STAT activity is required to induce tumorigenesis of nTSG-knockdown cells.
(A) Normal wing imaginal disc with the JAK/STAT activity reporter, 10xSTAT-GFP (green). Distal (D.f.), medial (M.f.) and proximal (P.f.) folds of the dorsal hinge are indicated. (B) Vertical section along the AP boundary of a wing disc with 10xSTAT-GFP (green) stained for adherens junction component Armadillo (magenta) (top panel). Lower panel: black line drawings trace the apical and basal sides of the epithelial layer. (C) A mosaic wing disc with clones expressing lgl-RNAi and GFP 7 d after RNAi induction. (D) Quantified occurrence ratio of tumorigenesis induced by scrib or lgl knockdown in the dorsal hinge region. (E) pJNK staining (magenta) in a third instar wing disc with ubiquitous lgl-RNAi expression since early second instar. The expression of lgl-RNAi is spatiotemporally controlled by act-Gal4 and temperature-sensitive (ts) Gal80 (Gal80ts). 10xSTAT-GFP, green. Nuclei were labeled with DAPI (blue) in (A), (C), and (E). White dotted lines mark the boundaries between the wing pouch and hinge regions in (A), (C), and (E). Scale bars represent 100 μm.