Cofilin1 Controls Transcolumnar Plasticity in Dendritic Spines in Adult Barrel Cortex
Fig 1
L2/3- and D2 column-restricted miRNA expression achieved through focal lentiviral injection.
(A) The impact of CFL1 on barrel map plasticity was examined through focal CFL1 KD within L2/3 of the D2 column. (B) The lentiviral vectors employed in this study for co-expressing either miRNA against CFL1 (miR-CFL1_1 or miR-CFL1_2) or miR-Neg and ChR2-eYFP. LTR, long terminal repeat. (C) A schematic diagram of intrinsic signal optical imaging of the right barrel cortex during left D2 whisker stimulation. (D, E) A vascular pattern image (D) and an intrinsic signal induced by D2 stimulation (E) during a representative imaging session. (F) An eYFP fluorescence image of the same cortical area shown in (D and E). Scale bar, 500 μm. (G, H) Tangential sections corresponding to L4 (G) and L2/3 (H) of a rat that was injected lentivirus into the D2 column. The L4 section was stained for cytochrome oxidase to visualize the barrel pattern that was projected onto the L2/3 image based on the vascular pattern. Scale bar, 500 μm. (I) A NeuN-stained coronal section of a virus-injected rat. Scale bar, 300 μm. (J) Magnified images of L2/3 (top row) and L5 (bottom row) of a MAP2 stained coronal section. Scale bar, 20 μm. (K) Percentage of eYFP+ neurons in each cortical layer. n = 5 injections.