Developmental Programming Mediated by Complementary Roles of Imprinted Grb10 in Mother and Pup
Figure 1
Comparison of Grb10KO and Grb10Δ2-4 mice.
(A) Structure of Grb10, according to UCSC annotation, showing numbered exons (boxes) and translated regions (filled boxes). The integrated gene-trap cassettes include splice acceptor (SA) and polyadenylation (pA) signals, and a LacZ reporter. (B) Comparative LacZ staining of bisected embryos at e14.5 inheriting the Grb10KO and Grb10Δ2-4 alleles through each of the parental lines. CNS expression observed in Grb10KO+/p embryos is not detected in Grb10Δ2-4+/p embryos. (C) Comparative LacZ staining of adult mammary glands at days 7.5 and 12.5 of gestation (G7.5, G12.5) and day 6 of lactation (L6), showing pregnancy-dependent reporter expression in Grb10KOm/+ but not Grb10Δ2-4m/+ females. WT (+/+) glands were stained with carmine alum to illustrate morphological changes.