A Force-Activated Trip Switch Triggers Rapid Dissociation of a Colicin from Its Immunity Protein
Figure 8
The effect of pulling geometry on the dynamic force spectrum of E9:Im dissociation.
(A) Dynamic force spectrum of E9:Im9 when extended from residues 3 (black circles and line), 30 (ochre circles and line), and 108 (red circles and line) on E9 and residue 81 on Im9. (B) Dynamic force spectrum of E9:Im9 when extended from residue 3 on E9 and residue 38 (green circles and line) or 81 (black circles and line) on Im9. Error bars are based on the standard deviation of measurements from triplicate datasets taken at each retraction velocity. Forces below the detection limit of the instrument and filtering software (18 pN, dashed line) are coloured grey. The pulling positions (yellow filled circles) relative to the binding interface is shown schematically (inset). For clarity, only the outer barrier that is rate limiting at low loading rates is plotted.