Structural Basis of Response Regulator Inhibition by a Bacterial Anti-Activator Protein
Figure 4
In vitro binding of wild-type RapF to His-ComA mutants targeting the RapF-ComA interface.
(A) RapF (blue surface) in complex with ComAC (brown cartoon). (B) Expanded view of the area enclosed by the black rectangle in panel A. (C) RapF interaction with the principal ComA DNA binding helix α9 (brown helix). (D) The binding of wild-type RapF to His-ComA mutants corresponding to the residues depicted as magenta sticks in panels B and C was determined by native PAGE.