Memory Phenotype CD4 T Cells Undergoing Rapid, Nonburst-Like, Cytokine-Driven Proliferation Can Be Distinguished from Antigen-Experienced Memory Cells
Figure 3
Effect of anti-MHC class II antibody (Y3P) on the proliferation of MP cells. (A)
2×106 CD45.1 OT-II CD4 T cells were injected IP into CD45.2 FcγRγ−/− B6 mice. 24 h later, mice were treated with Y3P (1.8 mg IP) or mouse immunoglobulin G (IgG), and 1 d later they were immunized IP with ovalbumin peptide (10 µg) plus LPS (25 µg) or LPS only. BrdU was given in drinking water from the time of immunization; 3 d later, lymph node cells were collected and stained with anti-CD45.1, anti-CD45.2, anti-CD44, anti-BrdU, and anti-Ki67. Contour plots were used because of the low number of cells available for analysis from mice treated with Y3P. (B) Numbers of CD45.1 and CD45.2 and of Ki-67+ BrdU+ cells from immunized control or Y3P-treated mice. (C) FcγRγ−/− B6 mice were treated for 3 d with 1.8 mg of Y3P followed by a 6-h BrdU pulse (1 mg). Numbers in the quadrants represent the frequency of BrdU-positive and -negative and Ki-67-positive and -negative cells for an individual animal. Lower panels present means and SDs for numbers of BrdU+ and Ki-67+ cells among the three animals in each group.