A Role for Myosin VI in the Localization of Axonal Proteins
Figure 11
Interaction with Myosin VI promotes enrichment of ChR2 at the surface of axons of layer 2/3 pyramidal neurons.
(A) Image of an acute brain slice with a recoding electrode. Cortical layers (L1 to L6) and white matter (WM) are indicated on the left. Blue grid represents 8×16 photostimulation patterns, with a spacing of 50 µm. (B) Schematic of waveforms triggered in soma and dendrites (red) and axons (blue). The arrow marks the clear inflection point (see Materials and Methods). (C) Example maps from a ChR2-MVIBD-GFP-positive cell (left) and a ChR2-GFP-positive cell (right). Black triangles mark soma locations. Blue traces indicate axonal excitation and red traces indicate somatic/dendritic excitation, respectively, using inflection point criteria. (D) The ratio of the number of APs evoked in soma and dendrites to the number of APs evoked in axons plotted against the laser power used for photostimulation. Blue circles represent the data points of the ChR2-MVIBD-GFP cells (n = 6), and red circles represent the data points of the ChR2-GFP cells (n = 8). The upward shift of the eDAR distribution of ChR2-MVIBD-GFP relative to that of ChR2-GFP is consistent with enrichment in axons of ChR2-MVIBD-GFP relative to ChR2-GFP.