Light Directs Zebrafish period2 Expression via Conserved D and E Boxes
Figure 11
Adjacent E- and D-boxes confer LRM mediated transcription.
PAC-2 cells were transiently transfected with a series of heterologous, E- and D-box-containing, luciferase reporter constructs. Transfected cells were then exposed to various lighting conditions as indicated by the black/white bars above the panels, and bioluminescence was monitored using a live-cell automated bioluminescence assay. Transfection results from heterologous constructs incorporating alternating E- and D-boxes (4xE/D-box) as well as multimerized D-boxes (6xD-box) and E-boxes-(4xE-box) are presented. Normalized luciferase activity (%) is plotted on the y-axis of each graph. Time (days) is plotted on the x-axis.