Direct Binding of pRb/E2F-2 to GATA-1 Regulates Maturation and Terminal Cell Division during Erythropoiesis
Figure 4
GATA-1, FOG-1, and pRb/E2F-2 interplay regulates proliferation.
(A) Cellular proliferation of eGFP-positive NIH 3T3 cells transduced with an “empty” retroviral vector (Migr) or retroviral vectors encoding hGATA-1, hGATA-1Rb−, hGATA-1V205G, or hGATA-1V205GRb−. We verified that >90% cells were successfully transduced by each of the retroviral vectors on the basis of coexpression of eGFP from an IRES. Cell proliferation was monitored each day for 5 d by Uptiblue. To increase legibility, curves are numbered 1 to 6 as well as represented with different colors. (B) Same as in (A), but using the G1E cell line. Because of the lower retroviral transduction efficiency of G1E cells (∼30%), we first sorted eGFP-positive cells for each of the retroviral vectors used on the basis of coexpression of eGFP from an IRES and then verified that the sorted population was >95% eGFP positive. To increase legibility, curves are numbered 1 to 6 as well as represented with different colors. (C) Same as in (B), but G1E cells were transduced with a retroviral vector (Migr) that expresses GATA-1s , which comprises a deletion of the first 83 amino acids.