A Cell Cycle Timer for Asymmetric Spindle Positioning
Figure 5
The Time of Anaphase Onset Does Not Depend on Spindle Displacement
(A) Time of NEBD (0 s) and soon after anaphase onset (189 s) in wild-type, par-2(RNAi), and gpr-1/2(RNAi) embryos. Yellow arrows mark 50% embryo length. par-2(RNAi) and gpr-1/2(RNAi) spindles did not become displaced toward the posterior. Anaphase began at roughly the same time as in wild-type.
(B) Quantitative analysis of time from NEBD to anaphase onset in wild-type and for embryos in which spindle displacement did not occur, in par-2(RNAi) and gpr-1/2(RNAi). Anaphase onset timing in par-2(RNAi) and gpr-1/2(RNAi) embryos were statistically indistinguishable from anaphase onset timing in wild-type embryos (p = 0.48 and p =0.33, respectively). All embryos express H2B:GFP and gamma-tubulin:GFP, and only embryos that divided equally after RNAi were analyzed. Number of cases analyzed for each experiment is indicated in parentheses.