Notch Promotes Neural Lineage Entry by Pluripotent Embryonic Stem Cells
Figure 1
R26NotchIC Does Not Impair ES Cell Self-Renewal
(A) RT-PCR for Notch receptors and ligands in Oct4-GiP ES cells selected in puromycin (“purified ES cells”). E13.5 neural tissue was used as a positive control.
(B) FACS analysis of Jagged1 expression in live unpermeabilised 46C ES cells using single-chain antibody to Jagged1. NS cells [42] were used as a positive control and a single-chain antibody to the irrelevant intracellular antigen Desmin was used for the negative control.
(C) FACS analysis of CD2 expression in R26NotchIC cells with parental 46C ES cells as controls.
(D–F) Analysis of R26NotchIC ES cells or parental control 46C ES cells after 10 to 12 passages in LIF+ serum. (D) R26NotchIC ES cells under phase contrast or stained for ES cell markers as indicated (average of three independent samples). (E) Quantitative RT-PCR analysis of ES cell markers, normalised to GAPDH, and displayed relative to expression in 46C ES cells. (F) FACS analysis of Sox1GFP expression.