Two-Photon Imaging of Cortical Surface Microvessels Reveals a Robust Redistribution in Blood Flow after Vascular Occlusion
Figure 4
Neuronal Integrity near Photothrombotic Clot
The animal was sacrificed 1 h after the disruption, and the tissue was harvested from below the target vessel shown in Figure 5C.
(A) The section, immunolabeled with an antibody against MAP2, shows widespread staining of neurons.
(B) Fluorescent imaging of propidium iodide counterstain. The stained endothelial cells demarcate vessels. Note the numerous nuclei with segmented lobes characteristic of aggregates of leucocytes within the vessel (clot).
(C) High-magnification view shows subtle neuropathology in some cells, i.e., corkscrew dendrites (single arrows) and shrunken neurons with eccentric nuclei (double arrow).