Bcl-w Enhances Mesenchymal Changes and Invasiveness of Glioblastoma Cells by Inducing Nuclear Accumulation of Β-catenin

Bcl-w a pro-survival member of the Bcl-2 protein family, is expressed in a variety of cancer types, including gastric and colorectal adenocarcinomas, as well as glioblastoma multiforme (GBM), the most common and lethal brain tumor type. Previously, we demonstrated that Bcl-w is upregulated in gastric cancer cells, particularly those displaying infiltrative morphology. These reports propose that Bcl-w is strongly associated with aggressive characteristic, such as invasive or mesenchymal phenotype of GBM. However, there is no information from studies of the role of Bcl-w in GBM. In the current study, we showed that Bcl-w is upregulated in human glioblastoma multiforme (WHO grade IV) tissues, compared with normal and glioma (WHO grade III) tissues. Bcl-w promotes the mesenchymal traits of glioblastoma cells by inducing vimentin expression via activation of transcription factors, β-catenin, Twist1 and Snail in glioblastoma U251 cells. Moreover, Bcl-w induces invasiveness by promoting MMP-2 and FAK activation via the PI3K-p-Akt-p-GSK3β-β-catenin pathway. We further confirmed that Bcl-w has the capacity to induce invasiveness in several human cancer cell lines. In particular, Bcl-w-stimulated β-catenin is translocated into the nucleus as a transcription factor and promotes the expression of target genes, such as mesenchymal markers or MMPs, thereby increasing mesenchymal traits and invasiveness. Our findings collectively indicate that Bcl-w functions as a positive regulator of invasiveness by inducing mesenchymal changes and that trigger their aggressiveness of glioblastoma cells.


Introduction
Bcl-w (B cell lymphoma-w), is expressed in a variety of cancer types, including GBM and colorectal adenocarcinomas, as well as gastric cancers [1]. Above all, GBM is difficult to treat using the conventional therapeutic options of standard surgical resection, radiation and chemotherapy, owing to its high frequency of recurrence [2], as well as is related to the upregulation of Bcl-w [3], MMP-2 (matrix metalloproteinase-2) [4][5][6][7] and β-catenin [8]. This cancer type is highly proliferative and exhibits mesenchymal characteristics, leading to tumor progression through acquisition of invasive or metastatic potential. Growing evidence suggests that Bcl-w enhances not only survivability as a pro-survival member of the Bcl-2 (B cell lymphoma-2) protein family [9][10][11], but also the migratory and invasive potentials of cancer cells as an additional function. In an earlier investigation, we reported that Bcl-w enhances migratory and invasive potential in gastric cancer cells [12,13].
Additionally, nuclear accumulation of β-catenin is frequently observed in invasive cancer cells, which modulates downstream targets contributing to cancer stemness and malignancy by binding to TCF (T-cell factor) and LEF (lymphoid enhancer factor) in the nucleus [14]. However, there has been no currently available information about relationships between glioma cell characteristics and upregulated proteins, such as Bcl-w, MMP-2 and β-catenin. Based on the current findings, we conclude that Bcl-w is critical for malignancy by functioning as a positive regulator of mesenchymal traits and invasion, and contribute significantly to a more comprehensive understanding of the tissue-specific role of Bcl-w in GBM.

Western blot analysis
Proteins either in conditioned media or in cell lysates prepared using a previously described method [15] were separated by SDS-PAGE, and electrotransferred to Immobilon membranes (Millipore, Bedford, MA), which were subsequently blotted using the indicated antibodies and visualized by the ECL detection system (Amersham, Uppsala, Sweden).

Invasion assays
These assays were conducted as described previously [12]. In brief, to compare invasiveness, cells (2 × 10 5 ) in 200 µl of medium were seeded onto the upper surfaces of Matrigelcoated polycarbonate filters which were placed in modified Boyden chambers (Corning, Corning, NY) that contain ECM components (BD Biosciences, Bedford, MA). The lower compartments of the chambers were filled with 1 ml of serumfree media supplemented with 0.1% BSA. After 20-24 hours of incubation at 37 o C, the cells that had migrated to the lower surface of the filter were fixed and stained using a Diff-Quick kit (Fisher Scientific, Pittsburgh, PA), then counted under a microscope (Mitoti AE31 series, Trinocular inverted MIC) [16]. The results were analyzed for statistical significance using the Student's t-test. Differences were considered to be significant at p< 0.05.

Wound healing assays
Cell were harvested with buffered EDTA and plated into 12well plates (6 × 10 4 cells/well). The confluent monolayer were scratched after 24hours and then allowed to migrated for 24hours at 37°C. Cell in five fields in the scratched area (200 x 500 µm 2 area) were counted under a light microscope (Mitoti AE31 series, Trinocular inverted MIC). Results were analyzed for statistical significance using Student's t test. Differences were considered significant at p< 0.05.

Gelatin zymography
This method was employed in order to analyze the activities of secreted MMPs. Conditioned media were prepared by incubating cells in serum-free media for 24 hours. Where indicated, the media were supplemented with specific inhibitors. Equal volumes of conditioned media were then subjected to 8% SDS-PAGE containing 0.1% gelatin. The gels were stained, and the MMPs activities were visualized as clear bands [16].

Cell fractionation
After washing cells with PBS, cells gently suspend in buffer A (containing 10mM HEPES (pH 7.9), 10mM KCl, 3mM MgCl 2 , 0.5% NP-40 and protease inhibitors). Lyse the cells on ice for 30 minutes, centrifuge at 10000 rpm for 5 minutes. Repeat centrifugation of supernatant and take supernatant for cytoplasmic fraction. Wash the pellet with cold PBS twice and break nuclei in buffer B (containing 20mM HEPES (pH 7.9), 400mM NaCl, 3mM MgCl 2 , 0.2mM EDTA and 25% glycerol) on ice for 30 minutes. After centrifuge at 13000 rpm for 15 minutes, take the supernatant (nuclear fraction) apart from the insoluble pellets.

Bcl-w is upregulated in glioblastomamultiforme tissues
Notably, Bcl-w expression was upregulated in GBM tissues (WHO grades IV), compared with normal and glioma grade III tissues in all patients examined ( Figure 1A).

Bcl-w positively regulates mesenchymal-related proteins
Epithelial-mesenchymal transition (EMT), characterized by loss of cell-cell adhesion, cytoskeleton rearrangement and manipulation of mesenchymal properties, is one of the crucial steps in cancer development, resulting in acquisition of the ability to metastasize. PI3K (phosphoinositide 3-kinase)/Akt and β-catenin/TCF-4 signaling pathways have been reported to play important roles in EMT and cancer progression [17][18][19]. In view of these data, we hypothesized that Bcl-w upregulates mesenchymal-related genes and plays a crucial role in acquisition of mesenchymal traits via stimulation of the PI3K-Akt-β-catenin-TCF-4 pathway.
Initially, we examined the expression levels of mesenchymal markers (vimentin, Twist1, Snail and Slug) in U251 glioblastoma cells via overexpression or depletion of Bcl-w with RNA interference ( Figure 1B, C). Immunoblot analysis revealed that expression levels of vimentin, Twist1, Snail and Slug were markedly increased in Bcl-w-overexpressing cells, compared with control cells ( Figure 1B). Bcl-w-targeted siRNA induced a significant decrease in levels of the mesenchymal protein markers vimentin, Twist1, Snail and Slug ( Figure 1C). Immunofluorescence analysis additionally disclosed a marked increase in the expression of vimentin ( Figure 1D) in Bcl-w overexpressing cells, compared with control cells. Other mesenchymal markers, N-cadherin and Claudin-1, were also increased in Bcl-w-overexpressing cells (data not shown). These results clearly demonstrate that Bcl-w promotes the expression of mesenchymal marker proteins, and thus contributes to the mesenchymal properties of GBM.

Twist1 and Snail contribute to Bcl-w-induced invasiveness
Treatment of control vector or Bcl-w-expressing cells with a PI3K inhibitor (LY294002, 10 µmol/L) and Akt inhibitor (10 µmol/L) led to a significant decrease in Twist1, Snail and vimentinl levels ( Figure 2A). Our data suggest that Bcl-winduced mesenchymal marker proteins are regulated by PI3K-Akt-β-catenin signaling.
Treatment of control or Bcl-w overexpressing cells with Twist1 or Snail siRNA effectively decreased vimentin expression ( Figure 2B) and Bcl-w-induced invasiveness ( Figure  2C).

Bcl-w promotes migration and invasion in U251 glioblastoma cells
We reported previously that Bcl-w enhances the migratory and invasive potential as well as survivability in gastric cancer cells [12,13]. Bcl-w expression has been positively associated with invading populations of cancer cells in GBM as well as gastric cancer. To establish the effects of Bcl-w on invasiveness and migratory ability in GBM, the protein was overexpressed in U251 cells ( Figure 3A). Bcl-w-expressing cells displayed enhanced migration and invasion, as observed with the wound healing and invasion assay using Matrigelcoated polycarbonate filters, respectively ( Figure 3B). To further ascertain the role of Bcl-w in invasiveness of U251 cells, the protein level was selectively reduced using two small RNA interference agents (siRNAs; si-Bcl-w-1 and si-Bcl-w-2). The Bcl-w-induced invasive ability of cells treated with these targeted siRNAs was effectively attenuated ( Figure 3C).

Bcl-w enhances the nuclear translocation of β-catenin
We further investigated the molecular mechanisms underlying Bcl-w-induced cell migration and invasion. To this end, we showed that Bcl-w stimulates p-Akt, p-GSK-3β (glucose synthesis kinase-3β), β-catenin, TCF-4 and MMP-2 levels or activities using Western blotting and zymography analyses, respectively ( Figure  4A). In particular, immunofluorescence and cell fractionation assays revealed phosphorylation of GSK-3β in Bcl-w-overexpressing cells in association with significantly enhanced nuclear accumulation of β-catenin, compared with control vector-transfected cells ( Figure 4B, C). Based on these data, we suggest that Bcl-w regulates positively nuclear β-catenin translocation and its binding to TCF-4 in the nucleus, in turn, stimulating the expression of target genes, such as MMP-2 [20,21] to promote migration and invasiveness in U251 glioblastoma cells.

PI3K and Akt act upstream of β-catenin in Bcl-winduced invasiveness
PI3K-Akt signaling is a crucial regulator of tumor cell invasion, growth, proliferation, survival, metabolism and apoptosis under different experimental conditions [22]. Aberrant activation of the PI3K-Akt pathway and genetic alterations of its components contribute to tumorigenesis [23]. Notably, activation of PI3K-Akt signaling has been reported in 84% of GBM samples [24].
To establish whether Bcl-w is linked to β-catenin via the PI3K-Akt pathway, control vector or Bcl-w-expressing cells were treated with an Akt inhibitor (10 µmol/L) and the PI3K inhibitor (LY294002, 10 µmol/L). The pharmacological inhibitors efficiently suppressed the expression of p-GSK3β, β-catenin, TCF-4 and MMP-2 proteins in cells displaying Bcl-w overexpression ( Figure 5A), as well as Bcl-w-induced cell invasion ( Figure 5B). Furthermore, siRNAs targeting β-catenin and TCF-4 induced a decrease in the MMP-2 and p-FAK (focal adhesion kinase) levels ( Figure 5C), along with invasive potential ( Figure 5D) of U251 cells. Our results indicate that PI3K and Akt act upstream of β-catenin in Bcl-w-induced glioblastoma cell invasion.

MMP-2 and FAK signaling components are involved in Bcl-w-induced invasion
MMP-2-targeted siRNA markedly reduced phosphorylation of FAK and Bcl-w-induced invasiveness ( Figure 6A). Moreover, suppression of FAK activity using RNA interference or dominant-negative mutants (FAKY397F) abolished Bcl-wstimulated invasiveness ( Figure 6B, C). Meanwhile, MMP2 levels are not changed by suppression of FAK. These data suggest that MMP-2 acts upstream of FAK in Bcl-w-induced glioblastoma invasion.

Bcl-w promotes invasion in several cancer cell types
To determine whether Bcl-w plays an invasion-promoting role in general, we examined whether the protein enhances p-GSK3β, β-catenin and MMP-2 expression in several types of cancer cells, including U373, U87MG (glioma), MDA-MB-231 (breast cancer) and H1299 (lung cancer). Transient transfection with a control and Bcl-w-overexpressing vector was performed in various cancer cells. Overexpression of Bclw enhanced β-catenin and MMP-2 levels significantly through phosphorylation of GSK3β. Moreover, Bcl-w-overexpressing cells displayed elevated invasive ability (more than 1.5 to 3 fold), compared to those transfected with the control vector ( Figure 7). To confirm these data using Bcl-w targeting siRNA, depletion of Bcl-w abolished invasive potentials in several cancer cells ( Figure S2).
These results suggest that Bcl-w enhances the invasive potential and mesenchymal properties of glioblastoma cells via nuclear β-catenin accumulation, including activation of MMP-2 and FAK or expression of vimentin via activation of Twist1 and Snail, respectively ( Figure S3).

Discussion
We have previously reported that Bcl-w is upregulated in gastric cancer cells which has infiltrative morphology and enhances migratory and invasive potential in gastric cancer cells by inducing the production of several types of extracellular matrix (ECM)-degrading proteinases [12,13]. These reports propose that Bcl-w is strongly associated with aggressive characteristic of GBM which has invasive and mesenchymal phenotypes. However, there is no information from studies of the role of Bcl-w in GBM. To investigate the tissue-specific role of Bcl-w in GBM, data obtained from all the patients indicate that Bcl-w expression is upregulated in GBM tissues (WHO grades IV), compared with normal and glioma grade III tissues ( Figure 1A). These results are supported by data that Bcl-w is upregulated in glioma tissues [3]. Recent reports have shown Conditioned media were prepared by incubating the vector and Bcl-w transfectants in serum-free medium for 24 hours. MMP-2 and MMP-9 activities were compared using zymography. Protein loading volumes were verified with Ponceau S staining. B, levels of βcatenin protein that translocated into the nucleus and Bcl-w protein in vector-or Bcl-w-transfected U251 cells were examined using confocal microscopy. Cells were stained with anti-β-catenin (green) or anti-Bcl-w (red) antibody, followed by nuclear staining with DAPI (blue). Scale bar, 50 µm. C, after separation of cells into cytoplasm and nuclear fractions for the indicated transfectants, each fraction was subjected to Western blotting with anti-β-catenin, anti-Lamin A/C (nucleus marker) and anti-β-actin (cytoplasm marker) antibodies. doi: 10.1371/journal.pone.0068030.g004 Figure 5. Bcl-w-induced β-catenin signaling components promote invasive potential via activation of the PI3K-Akt pathway. A, the indicated U251 cell transfectants were incubated in serum-free medium in the presence or absence of PI3K inhibitor (LY294002 (LY); 10 µmol/L) or Akt inhibitor (Akt-I; 10 µmol/L) for 1 hour. Expression levels and activities of p-Akt, p-GSK3β, β-catenin, TCF-4 and MMP-2 proteins were compared using Western blotting. B, cells treated with PI3K inhibitor or Akt inhibitor in the lower compartments of the invasion chambers for 24 hours, respectively. Invasive potential of treated cells was compared. *, p< 0.05 versus untreated control, n = 5. C, β-catenin and TCF-4 siRNAs (20nM) were introduced into vector or Bcl-w overexpressing cells, and cellular levels of β-catenin, TCF-4, MMP-2 and p-FAK compared after 24 hours of incubation using Western blotting with β-actin as a loading control. D, invasive potential of the indicated transfectants was compared. *, p< 0.05, n = 5. that glioblastoma cells have features of mesenchymal traits and invasiveness resulting in the tumor progression [25,26]. Loss of E-cadherin and gain of vimentin are considered major events, and Twist1, Snail and Slug are crucial transcription factors involved in the EMT process [27,28]. Twist1 enhances glioma invasion in concert with mesenchymal changes [29]. SNAI2/Slug is overexpressed in glioma cell lines and promotes invasion and growth in human gliomas [30]. SNAI1/Snail is involved in proliferation and migration of glioblastoma cells [31]. Our results show that Bcl-w promotes the expression of Twist1,  Figure  1B). Immunofluorescence analysis additionally disclosed a marked increase in the expression of vimentin ( Figure 1D) in Bcl-w overexpressing cells, compared with control cells. To confirm these data, transfection of U251 glioblastoma cells with Bcl-wtargeted siRNA significantly suppressed expression of the mesenchymal markers, Twist1, Snail, Slug and vimentin ( Figure 1C), and reduced invasive ability via inhibition of MMP-2 production ( Figure 6A). Similarly, Bcl-2 overexpression has been reported to induce partial EMT and promote squamous carcinoma cell invasion and metastasis [32]. Our data suggest that Bcl-w-induced mesenchymal marker proteins are regulated by PI3K-Akt-β-catenin signaling by treatment of PI3K inhibitor or Akt inhibitor (Figure 2A). Consistent with the theory that Bcl-w-induced signaling plays a key role in EMT events, depletion of Twist1/Snail led to reduced Bcl-w-induced vimentin expression and invasion, further confirming the association between mesenchymal factors and Bcl-w-induced invasive signaling ( Figure 2B, C). Vimentin knockdown additionally inhibited invasion of U251 glioblastomas ( Figure  S1). These results provide strong evidence that Bcl-w reinforces mesenchymal changes as an important modulator of malignant progression in glioblastoma cells. We further reported that upregulation of Bcl-w is associated with properties of GBM (such as mesenchymal traits and invasiveness), since Bcl-w overexpression increased migratory potential and invasiveness in glioblastoma U251 cells ( Figure  3A-C). Previously, we demonstrated that Bcl-w-induced migratory and invasive properties are accompanied in gastric cancer cells [12,13]. However, our recent experiments have important meaning because of study about the role of Bcl-w related in the feature of this tissue. Bcl-w overexpression of GBM cells led to elevation of the levels of p-Akt p-GSK3β, βcatenin, TCF-4 and MMP-2 proteins ( Figure 4A). Meanwhile, our previous study had showed that Bcl-w-induced Sp1 expression contributed to invasiveness by activating MMP-2 in gastric cancer cells. Therefore, we also investigated that Sp1 involved Bcl-w-induced invasion signaling in glioblastoma cells (data not shown; in submission). β-Catenin, such as another transcription factor which focused in this study, is a multifunction protein that participates in cell-cell interactions between E-cadherin and the cytoskeleton, and acts as an upstream regulator of TCF/LEF transcription factors. The constitutively activated Wnt/β-catenin signaling pathway is a key regulator in tumorigenesis and cancer malignancies [33][34][35], in particular, human gliomas [36,37]. Data from the immunofluorescence assay and cell fractionation using western blot analysis revealed that Bcl-w stabilizes the β-catenin level and facilitates its subcellular localization into the nucleus ( Figure 4B, C) instead of cytoplasmic β-catenin degradation via the ubiquitin-proteosome system through formation of Axin-GSK3β-APC (adenomatous polyposis coli) complexes, by participating in the Wnt/β-catenin pathway. In the nucleus, βcatenin binds to LEF/TCF family of transcription factors, which may play a crucial role in the Wnt signaling pathway to drive the transcription of target genes, including cell proliferation, differentiation, and migration [38][39][40]. Our data suggest that Bcl-w-induced invasion of glioblastoma cells is related to the βcatenin/TCF-4 pathway that acts as a downstream regulator of PI3K and Akt ( Figure 5A-D). Thus, β-catenin appears to participate in the invasion process. In addition, small interfering RNA against MMP-2 diminished activation of FAK and Bcl-winduced invasion ( Figure 6A). Finally, ablation of FAK activation with the use of siRNA or a dominant-negative mutant (Y397F) decreased invasion ( Figure 6B, C). Meanwhile, MMP2 levels are not changed by depletion of FAK using siRNA or dominant negative mutant. These data suggest that MMP-2 acts upstream of FAK in Bcl-w-induced invasion of U251 cells. These data were supported previous data of gastric cancer cells, SNU484, that Bcl-w induced migratory and invasive potentials were enhanced the phosphorylation of FAK by activating of MMP-2 or uPA (urokinase plasminogen activator) [12,13].
Therefore, the invasion of glioblastoma stimulated by Bcl-w is mediated by a cellular signaling pathway that involves the translocation of nuclear β-catenin via activation of PI3K/Akt and sequential upregulation of MMP-2 and FAK signaling components. The promotion of invasive ability by Bcl-w appears to be a general phenomenon that occurs via similar signaling pathways in various cancer cell types (Figure 7, S2).
In summary, high expression of Bcl-w is clearly associated with mesenchymal changes and invading populations in the glioblastoma multiforme ( Figure S3). Comprehensive investigation has revealed that Bcl-w functions as a positive regulator of invasion by enhancing mesenchymal traits of GBM, consequently contributing to malignancy. The findings of this study shed further light on the specific role of Bcl-w related in the features of glioblastomas.