Seasonal, Diurnal and Vertical Variation of Chlorophyll Fluorescence on Phyllostachys Humilis in Ireland

In recent years, temperate bamboo species have been introduced in Europe not only as an ornamental plant, but also as a new biomass crop. To measure adaptation stress of bamboo to the climate of Western Europe, chlorophyll fluorescence was measured on a diurnal and seasonal basis in Ballyboughal, Co. Dublin, Ireland. Measurements were attained on the leaves of each node of Phyllostachys humilis. The most frequently used parameter in chlorophyll fluorescence is the photosynthetic efficiency (Fv/Fm). A seasonal dip-as well as a larger variation-of Fv/Fm in spring compared to the rest of the year was observed. Over the year, the upper leaves of the plant perform better than the bottom leaves. These findings were linked to environmental factors such as light intensity, air temperature and precipitation, as increased light intensities, decreasing air temperatures and their interactions, also with precipitation levels have an effect on the photosynthetic efficiency (Fv/Fm) in these plants. Copyright: ß 2013 Van Goethem et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The Special Research Fund (BOF) partly funded this research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study.


Introduction
Bamboo is a widespread woody grass with over 1200 species and 90 genera [1], covering large areas north and south of the Equator. However, like most woody plants, bamboos have failed to colonize the harshest environments, such as the arid desert or Arctic tundra. Due to the last ice age, Europe and most of North America were thus excluded from the potential areas where bamboos could establish. However, temperate bamboos are growing in these two continents and, if climatic history had been different, these plants may well have been native in Europe and North America [2].
Although some of them are extremely fast-growing, bamboo species have largely been neglected in the search for renewable energy sources [3][4][5]. However, despite the fact that they are not native to this continent, in recent years, there is a growing interest for these exotic plants in Europe [6]. On top of their potential as biomass producer, bamboo can also be used for renewable biobased materials such as wood, composites, vessels and chemicals [3,[7][8][9] and for the removal of pollutants -such as heavy metalsout of the soil [5].
The bamboo genera most suitable in Europe are temperate bamboos such as Sasa, Fargesia and Phyllostachys. Among these, Phyllostachys species are known to have a very high annual biomass production [3], and they can survive temperatures as low as 220uC [10]. These temperate bamboos can be cultivated in Europe, however, it is still unknown how these plants respond and develop in this foreign environment. To assess the possible damages of different (environmental) stress signals, fluorescence spectroscopy can be used [11].
The shape of the fluorescence transient of any sample is determined by the physiological state of the sample at that moment and the physical and chemical environmental conditions around the sample [12], such as light intensity [13][14][15], temperature [16][17][18], drought [19] or chemical influences [20]. A light-induced decline of photosynthetic activity is broadly termed as photoinhibition [21]. It is a process taking place when the photosynthetic apparatus receives excess excitation energy and is hence called the excitation pressure hypothesis [22][23][24]. The site of inhibition of photosynthesis is photosystem II [25] and photoinhibition characteristically causes a loss of variable chlorophyll fluorescence [26]. One of the most frequently used parameters in chlorophyll fluorescence is the ratio between variable and maximum fluorescence (Fv/Fm). Across a wide range of higher plants species, this parameter has an optimal value of 0.83. When exposed to abiotic and biotic stresses, Fv/Fm in plants will decrease [27]. This parameter is therefore frequently used as a stress detector under environmental stress conditions, such as light [28], temperature [24] and drought [29].
Seasonal dynamics in chlorophyll fluorescence have been reported for different species. Depressions in photochemical efficiency (Fv/Fm) were observed in winter in Mediterranean shrub species [30,31], during cold winter days in conifer species [32][33][34][35] or in spring and early summer in Mediterranean grassland species [36] when ambient temperatures were increasing rapidly and precipitation was low. Aside from seasonal changes, chlorophyll fluorescence can also vary between sun-exposed and shaded leaves [37][38][39][40][41]. Also midday depression of photochemical efficiency is common in higher plant species like in Mediterranean grassland species [42] and in other bamboo species [43].
The seasonal dynamics in chlorophyll fluorescence in other plants raise the hypothesis that the photochemical efficiency in Phyllostachys humilis in Ireland would be lower in winter or in spring and early summer compared to the rest of the year. Also a midday depression can be expected, as this is observed in other higher plant species. These variations in photochemical efficiency will probably be correlated with environmental factors such as light intensity, air temperature and precipitation. Since lower leaves are less exposed to microclimatic variations in the environment compared to upper leaves, we also hypothesize that the lower the leaf in the canopy, the higher the photochemical efficiency will be.
Therefore, the objective of this study was to investigate seasonal, diurnal and vertical variation of Fv/Fm, in relation to light intensity, air temperature and precipitation on the bamboo Phyllostachys humilis in Ballyboughal, Ireland.

Plant Material
In April 2005, a 5 m680 m strip of Phyllostachys humilis was planted at a distance of 2 m within rows and 1.8 m between rows on a clay soil in Ballyboughal, co, Dublin, Ireland (N 53u319410 E 6u159310). The land accessed was privately owned and the owner of the land gave permission to conduct the study on this site. No protected or endangered species were sampled.
We selected Phyllostachys humilis since it is a temperate bamboo species, well suited for a temperate climate such as this of Ireland, and for his high biomass production [44]. Plants were produced via in vitro techniques and delivered by Oprins Plant NV (Rijkevorsel, Belgium) and were planted after hardening during one growing season in a 2 litre pot. At the moment of planting, the average height was about 50 cm. No fertilizer was added. During the first two years after planting, the plants were regularly watered during summer and Simazineß was used to suppress upcoming weeds. Additional water or spraying with herbicides was unnecessary from the third year onwards. The nodes were numbered from top (NN = 1) to bottom (NN = 13), as we were interested in the vertical effect of depth in the canopy. The maximum node number sampled was 13. Measurements were taken with the Handy PEA (Hansatech instruments Ltd., England, Norfolk) after dark adaptation with leaf clips for 30 minutes. Biolyzer HP 3.0 (Fluoromatics Software) was used to calculate Fv/Fm as an indication of leaf photosynthetic performance.

Data Collection
In spring, summer and autumn of 2012, LAI-2000 measurements were performed under over-cast conditions as advised by [45] to reduce the effect of scattered blue light in the canopy. Above and below canopy measurements were performed at five randomly chosen locations in the study plot.
Besides with the LAI-2000 light intensity in the canopy was also measured with the TRAC (Tracing Radiation and Architecture in Canopies [46]). TRAC measurements were performed along 2 transects over 12 m oriented in an E-W direction (to avoid boundary effects of the edge of the field). All TRAC measurements were taken under clear sky conditions to allow uninterrupted operation. The operator always held the TRAC in balance when operating and walked with a reasonable constant speed, also advised by [45] The LAI (Leaf Area Index) was calculated as the mean of the LAI-values obtained via these two instruments, as recommended by [45] for an accurate LAI estimation.
Hourly values of global radiation, air temperature and precipitation for the period from 01/01/2009 till 31/12/2011 were available from Dublin Airport, less than 12 km distant from the field (Figure 1). Meteorological conditions were comparable over the three years, with maximum global daily variation values reached in early summer (25 to 30 MJ/m 2 day), mean daily air temperatures ranging from 0 to 27uC in winter and up to 17uC in summer, and a mean monthly precipitation of about 70 mm (Fig. 1).

Statistical Analysis
3.1. Seasonal, diurnal and vertical variation. In order to analyse the seasonal, diurnal and vertical variation, a statistical mixed model is used. Although harmonic functions are often used to model temporal data [47], the use of a nonlinear function is a better representative for describing Photosystem II (PSII) efficiency in the leaves of Phyllostachys humilis [48]. Therefore, data were analysed in the statistical program R v2.13.0 [49] using a nonlinear mixed model [50] with leaf nested in node nested in culm with the following function: We tested the effect of TOD (time of the day) and node number (NN) and the two-way interaction of both factors on this model. The effect of TOD was tested for three moments during the day: morning, midday and afternoon; with morning measurements taken between 8 am and 10 am, midday measurements between 12 pm and 2 pm, and afternoon measurements between 4 pm and 6 pm, all expressed in local time. Variance functions allowing for different standard deviations (for season as well as for node number) were used as described by Pinheiro & Bates (2000). They were both optimized based on the Akaike Information criterion (AIC) [51].

3.2.
Effect of light, air temperature and precipitation. Since the seasonal, diurnal and vertical effects are possibly correlated with environmental conditions, a linear mixed model [50] with leaf nested in node nested in culm was used to test the effect of light, air temperature and precipitation and their interactions on Fv/Fm. Since we expect the adaptation of the photosynthetic system to be rather slow [35], the mean air temperature and the sum of precipitation and global radiation were taken over the last 30 days prior to the measurement. Penetrated solar radiation at different node depths was estimated based on LAI-2000 measurements, assuming an exponential decrease in radiation with the depth in the canopy. Of each meteorological variable, the amount of variance accounted for in the population was estimated by the omega squared (V 2 ) [52].
Variance functions allowing for different standard deviations for season were used as described by Pinheiro & Bates (2000), optimized based on the Akaike Information criterion (AIC) [51].

LAI
Mean LAI of the bamboo canopy was found to be 7.261.7 m 2 m 22 , with no significant differences between the different seasons ( Table 1).

Seasonal, Diurnal and Vertical Variation
A depression in Fv/Fm can be clearly observed in spring, a recovery in summer and high values in autumn and winter, as  fitted by the nonlinear function ( Figure 2). Also the variance within each season showed large differences between seasons. The variance in Fv/Fm was small in summer, autumn and winter (var (Fv/Fm) = 0.0004, 0.0020 and 0.0026 resp.). Towards the end of the winter the variability in Fv/Fm increased (var (Fv/ Fm) = 0.0041), reaching maximum values in spring (var (Fv/ Fm) = 0.0187) (Figure 2). Also the variance of Fv/Fm in node number differs and is higher in the top leaves (var (Fv/ Fm) = 0.0109 in the leaves of the top 2 nodes) compared to the bottom leaves (var (Fv/Fm) = 0.0104 in the leaves of the bottom 2 nodes), and this is even more explicit in summer, autumn and winter than in spring. Node number has only an effect on the amplitude of the Fv/Fm values (parameter a in eq.1), while daytime has a significant effect on the amplitude of the Fv/Fm values, the width of the seasonal dip and the DOY min (parameters a, b, and d in eq. 1, respectively) ( Table 2). No significant effect was found on the minimum of Fv/ Fm (parameter c in eq.1).
Fv/Fm is lower in the top leaves than in the leaves more deeply in the canopy (the effect of NN on the amplitude of Fv/Fm (parameter a in eq.1) is a positive value, and this parameter represents higher values in summer, autumn and winter) (Table 3, Figure 3). Maximum Fv/Fm values (in summer, autumn and winter) are higher in the afternoon than in morning and midday, with no statistical significant difference between morning and midday (the magnitude of the effect of TOD pm on parameter a is 2-3% higher than the magnitude of the effect of TOD am and TOD noon on this parameter (Table 3). In spring, minimum values of Fv/Fm (parameter d of eq.1) are first observed for the morning measurements (DOY = 92) (Figure 4). For the midday and afternoon measurements, these minimum Fv/Fm values (parameter d) are only reached at DOY = 101 (Table 3). Also, the depression of the dip is less wide for the morning measurements compared to the midday and afternoon measurements (parameter b of eq.1 in table 3).

Effect of Light, Air Temperature and Precipitation
Only the effect of precipitation on the photochemical efficiency Fv/Fm was not significant (p = 0.45). All other effects (the effect of light intensity, air temperature and all two-way interaction) on the photochemical efficiency Fv/Fm were significant at p,0.001 (table 4). The negative estimated value of light means that increasing radiation intensity will result in a lower Fv/Fm-value. Air temperature has a positive effect on the chlorophyll fluorescence efficiency, as well as precipitation. The interaction  between light and air temperature is positive, meaning that the negative effect of an increasing radiation on Fv/Fm is less under increasing air temperature. The interaction between light and precipitation is negative, meaning that the negative effect of an increasing radiation will be higher when precipitation level is increasing as well, however, the size effect of this parameter is very low (V 2 = 0.004), meaning that this factor is not accounting for much of the explained variation. The interaction between air temperature and precipitation is also negative, meaning that the positive effect of high air temperature will lessen upon elevated precipitation levels.

General Effect of Light, Air Temperature and Precipitation
Since Phyllostachys humilis is a bamboo species native to the continental temperate climate of China [1], we did not expect the temperate weather condition of Ireland to be stressful for these plants. Intriguingly, we did found a significant effect of light, air temperature, precipitation and an interaction between these factors on chlorophyll fluorescence.
Air temperature has the largest effect on Fv/Fm (Table 4), with lower Fv/Fm values under decreasing air temperatures, indicating that decreasing air temperatures are stressful for the P. humilis plants. In literature, the negative effect of low air temperatures on Fv/Fm values has been previously reported in evergreen conifers, where reduced photochemical efficiency during winter was related to damage of the PSII reaction centers [39,[53][54][55].
Beside the effect of air temperature, we found that increasing light intensities reduces Fv/Fm in the leaves of P. humilis. Light stress is often referred to as an important stress factor that can induce photoinhibition [28,56,57]. When the amount of absorbed radiation exceeds the ability of the photosynthetic apparatus to use it, the surplus energy has to be quenched in order to avoid photooxidative damage to the thylakoid membranes [58]. In their   natural habitat, bamboo leaves are sun-protected, since many bamboo species are typical understory tree-grasses in Japanese, Chilean and Chinese temperate and subalpine forests [59][60][61][62][63][64].
Although mostly Sasa, Chusquea and Bashania are described as understory genera [65], P. humilis is also found to be originated in the temperate Chinese forests [1], which might explain its sensitivity for light. An important interaction was observed between light and air temperature, as the interaction amplifies the effect of these two factors. This means that the negative effect of increased light intensity on Fv/Fm is even stronger at decreasing air temperatures, whereas at increasing air temperatures, the negative effect of increasing light intensities will be less. Together with the main effects of light and air temperature, this is consistent with the excitation pressure hypothesis. Photoinhibition will take place when the photosynthetic apparatus receives excess excitation energy, and at low temperatures, this may occur under quite low light levels [22,23]. Although their size effect is lower (V 2 = 0.004 and 0.019, respectively) compared to the former interaction, the interactions between light and precipitation, and between air temperature and precipitation were also found to be significant. In combination with increased light intensities, and in combination with increased air temperature levels, the non-significant positive effect of precipitation on Fv/Fm will be lower, as under the climatic conditions of Ireland, light and temperature effects are more important than the precipitation effect as indicator of soil moisture deficit. However, in more precipitation limited regions, the relative importance of these effects might change.

Seasonal Variation
In the bamboo plantation, a clear seasonal depression of chlorophyll fluorescence was found in spring. This spring dip has also been found in Mediterranean grassland species [36]. The declined Fv/Fm values in spring might be explained by a combination of low air temperatures and increasing light irradiation ( fig. 1), as photoinhibition can occur under these conditions [35,38]. The increased quenching probably results from downregulation and increased zeaxanthin concentrations within the thylakoid membrane [66]. Another explanation might be that soil water availability is more important during culm development, which starts in spring [10], than in the rest of the year. As the soil water availability is linked with precipitation level, it might be the precipitation level in spring is too low, and can cause stress to the plants.

Diurnal Variation
Although midday depression has been found in other bamboo species like Guadua angustifolia and Bambusa tulda [43], no midday depression was observed in Phyllostachys humilis, just as in other bamboo species such as Dendrocalamus gigantus en D. strictus [43]. However, as observed by Kumar et al. (2002), Fv/Fm can be lower in the morning, with a recovery later during the day. In P. humilis, this effect was only found in early spring. In late spring, however, the reverse effect was observed ( fig. 4). The lower values of Fv/Fm in the morning might be explained by the lower air temperature in the morning compared to the noon and afternoon. The reverse effect (higher values in the morning compared to the rest of the day) can be explained by the recovery from photoinhibition as nights are warmer in late spring. Other grasses that are able to tolerate cold air temperature also show rapid photosynthetic recovery when air temperatures rise in spring [67].

Vertical Variation
The negative effect of increased light intensities on Fv/Fm is also reflected in the significant effect of the depth of the leaves in the canopy. The leaves more deeply in the canopy have a higher Fv/Fm than leaves located at the top. A similar effect has been reported in boreal conifers, where a higher proportion of damaged reaction centers was found in sun-exposed than in shaded needles [40,41,68,69]. Also in the evergreen shrub Mahonia repens, sunexposed leaves have a lower photosynthetic efficiency during winter, whereas shade leaves did not [70]. The deeper located leaves are more protected from the sun, due to the high LAI of Phyllostachys humilis of 7.261.7 m 2 m 22 . This LAI value is comparable with that of P. pubescens, where a value of 8.0 m 2 m 22 was measured [71]. For P. bambusoides [72] even observed a value of 11.6 m 2 m 22 . Therefore, in mature stands, transmission of light decreases rapidly from the top of the bamboo canopy towards the bottom [73]. In this way, the dense canopy protects the bottom leaves against increasing radiation.

Conclusions
We found that environmental factors as high light intensity and low air temperature can have a negative impact on Fv/Fm, representing the photochemical efficiency of the leaves of P. humilis, even in the temperate climate of Ireland. These effects of the environmental weather conditions explain the occurrence of a seasonal, diurnal and vertical variation in Fv/Fm of our data.
Notwithstanding the spring dip, the photochemical efficiency of the P. humilis leaves was generally not lower than what we would expect under optimal conditions (Fv/Fm was not much lower than 0.8). Therefore, we conclude that bamboo was not under environmental stress under these temperate conditions and thus, their potentials as a viable (biomass)crop can also be exploited in non-native European environments.
As the photochemical efficiency of the leaves is highest under shaded conditions, it might be interesting to use bamboo in agroforestry systems, by cultivating other plants (trees) above the bamboo canopy to higher the yields. However, in practice, Phyllostachys spp. are mostly cultivated in open, sunny situations [1]. Moreover, a higher photochemical efficiency does not necessarily mean a higher biomass production, since competition can arise respective to water use, and although a correlation with the maximum light-saturated photosynthetic rate is frequently observed [74][75][76], a low photochemical efficiency does not necessary result in a low photosynthetic activity. Therefore, gas exchange measurements should be executed to find out if high light intensities and low air temperatures and precipitation affect the overall photosynthetic activity in the same way as they influence Fv/Fm.