Interference of Quorum Sensing by Delftia sp. VM4 Depends on the Activity of a Novel N-Acylhomoserine Lactone-Acylase

Vimal B. Maisuria; Anuradha S. Nerurkar

doi:10.1371/journal.pone.0138034

Abstract

Background

Turf soil bacterial isolate Delftia sp. VM4 can degrade exogenous N-acyl homoserine lactone (AHL), hence it effectively attenuates the virulence of bacterial soft rot pathogen Pectobacterium carotovorum subsp. carotovorum strain BR1 (Pcc BR1) as a consequence of quorum sensing inhibition.

Methodology/Principal Findings

Isolated Delftia sp. VM4 can grow in minimal medium supplemented with AHL as a sole source of carbon and energy. It also possesses the ability to degrade various AHL molecules in a short time interval. Delftia sp. VM4 suppresses AHL accumulation and the production of virulence determinant enzymes by Pcc BR1 without interference of the growth during co-culture cultivation. The quorum quenching activity was lost after the treatment with trypsin and proteinase K. The protein with quorum quenching activity was purified by three step process. Matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) and Mass spectrometry (MS/MS) analysis revealed that the AHL degrading enzyme (82 kDa) demonstrates homology with the NCBI database hypothetical protein (Daci_4366) of D. acidovorans SPH-1. The purified AHL acylase of Delftia sp. VM4 demonstrated optimum activity at 20–40°C and pH 6.2 as well as AHL acylase type mode of action. It possesses similarity with an α/β-hydrolase fold protein, which makes it unique among the known AHL acylases with domains of the N-terminal nucleophile (Ntn)-hydrolase superfamily. In addition, the kinetic and thermodynamic parameters for hydrolysis of the different AHL substrates by purified AHL-acylase were estimated. Here we present the studies that investigate the mode of action and kinetics of AHL-degradation by purified AHL acylase from Delftia sp. VM4.

Significance

We characterized an AHL-inactivating enzyme from Delftia sp. VM4, identified as AHL acylase showing distinctive similarity with α/β-hydrolase fold protein, described its biochemical and thermodynamic properties for the first time and revealed its potential application as an anti-virulence agent against bacterial soft rot pathogen Pectobacterium carotovorum subsp. carotovorum based on quorum quenching mechanism.

Citation: Maisuria VB, Nerurkar AS (2015) Interference of Quorum Sensing by Delftia sp. VM4 Depends on the Activity of a Novel N-Acylhomoserine Lactone-Acylase. PLoS ONE 10(9): e0138034. https://doi.org/10.1371/journal.pone.0138034

Editor: Yogesh S. Shouche, National Centre for Cell Science, INDIA

Received: April 21, 2015; Accepted: August 24, 2015; Published: September 18, 2015

Copyright: © 2015 Maisuria, Nerurkar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: VBM acknowledges the University Grants Commission (New Delhi, India) for a meritorious student research fellowship (UGC sanction No. F.4-1/2006/XI plans/BSR) and Commonwealth Scholarship Commission in the United Kingdom for the Commonwealth split-site doctoral scholarship-2009. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Novel antimicrobials which specifically target the virulence factors are potentially revitalizing the pathogen management practices. Quorum sensing mechanism has been well studied in several gram-negative pathogens, such as Pectobacterium species, Pseudomonas aeruginosa and Vibrio cholarae [1,2]. Virulence gene expression in many Gram-negative pathogens generally is found to be under quorum sensing control [3–5]. In particular, quorum-sensing plays an important role in the regulation of virulence genes (mainly Plant Cell Wall Degrading Enzymes, PCWDEs production) in phytopathogenic soft rot causing Pectobacterium species. Also the N-acylhomoserine lactone (AHL) based quorum sensing in soft rot Pectobacterium species tightly regulates the activities implicated in microbial competition and host-pathogen interaction. These activities require various microbial functions such as biofilm formation, expression of virulence factors, antibiotic production etc. [6–11]. This mechanism of gene regulation could also presumably provide advantage to different competitors with quorum-sensing regulated functions in their natural surroundings.

Quorum sensing system is target of many antimicrobial strategies [12,13]. Enzymatic degradation of AHLs is generally the most common approach to quorum quenching [8,12,13,14]. Several soil bacteria producing AHL degrading enzymes have been characterized extensively for quorum quenching in past decade. Two major groups of AHL-degrading enzymes from bacterial species have been well characterized in literature [8,13]. One of which is AHL lactonase, a type of widely characterized AHL degrading enzymes, which was identified as aiiA in a Bacillus species and interferes with quorum sensing system of other bacteria by hydrolyzing the lactone ring of AHL [12,15–17]. Second type of AHL degrading enzyme found in many Gram-negative bacteria is AHL acylase which degrades the AHL signal molecules by hydrolyzing the amide linkage of AHL [18–20]. Bacterial system or in transgenic plants expressing AHL degrading enzymes show quorum quenching due to abolition of the quorum sensing regulated virulence and thereby infection [12,20]. The AHL acylase have advantage over AHL lactonase producing bacteria as the AHL degradation product of AHL acylase is homoserine lactone (HSL) and fatty acyl moiety that can be further metabolized as carbon, nitrogen and energy source by bacteria, while acyl homoserine, the product of AHL lactonase causes inhibitory effect on its growth to certain extent [15]. Altogether, studies have demonstrated that different AHL degrading enzymes of soil bacteria could efficiently counteract the quorum sensing regulation of bacterial pathogenicity and could be utilized as a new type of biocontrol mechanism [9,18,21,22].

It is well known that virulence of soft rot causing Pectobacterium carotovorum subsp. carotovorum (Pcc) is associated with the production of PCWDEs including pectate lyase, pectin lyase, polygalacturonase and cellulase [6,10,23]. Here in the present studies, an efficient AHL degrading soil isolate was evaluated for its interference on quorum sensing of soft rot causing Pcc strain BR1. The present study was undertaken with an aim of purification, identification and biochemical characterization of the AHL degrading factor.

Materials and Methods

Bacterial strains and culture conditions

All bacterial cultures were grown in their appropriate medium at 30°C under shaking condition with 140 rpm. Biosensor strain Chromobacterium violaceum CV026 was used to detect AHL [24,25]. Biosensor strain C. violaceum CV026 was grown overnight and maintained on 5.0 g L^-1 Tryptone, 3.0 g L^-1 Yeast extract (TY) medium supplemented with 20 μg mL^-1 Kanamycin at 30°C. The Escherichia coli JM109 [F′traD36 proAB lacIqlacZΔM15/recA1 endA1 gyrA96 thi hsdR17 supE44 relA1Δ (lac-proAB) mcrA] bioluminescence biosensor strains based on luxRI’ [pSB401 for N-hexanoyl-homoserine lactone (HHL, Sigma-Aldrich), N-3-oxo-hexanoyl-L-HSL (OHHL) and N-3-oxo-octanoyl-L-HSL (OOHL, Sigma-Aldrich)] and lasRI’ [pSB1075 for N-decanoyl-L-HSL (DHL, Sigma-Aldrich)] were used for AHL bioassay. These biosensor strains were grown and maintained in Nutrient Broth (NB per L: 5.0 g peptic digest of animal tissue, 5.0 g sodium chloride, 1.5 g beef extract, 1.5 g yeast extract and pH adjusted to 7.4 before sterilization) [26] amended with appropriate antibiotics (5 μg mL^-1 Tetracycline for E. coli JM109 pSB401 and 40 μg mL^-1 Ampicilin for E. coli JM109 pSB1075 at 30°C). As mentioned in previous studies the P. carotovorum subsp. carotovorum BR1 was grown and maintained on Nutrient broth supplemented with Pectin (NBP per L: 5.0 g peptic digest of animal tissue, 5.0 g sodium chloride, 1.5 g beef extract, 1.5 g yeast extract, and 5.0 g pectin, pH adjusted to 6.5 before sterilization) [27] at 30°C. AHL degrading bacterial isolates were initially grown on OHHL amended minimal medium and further maintained on NB.

Isolation, characterization, identification and growth condition of AHL degrading bacteria

Turf soil samples were collected from the gardens of Faculty of Science, The M.S. University of Baroda, India. The medium reported by Leadbetter and Greenberg [19] was used for the enrichment and isolation of AHL-degrading bacteria. Growth substrates (2 mM HHL) were added to the autoclaved, vitamin-amended medium as indicated. At the end of enrichment cycle, colonies with diverse cultural characteristics were screened for the AHL-degrading properties in AHL-bioassay with biosensor strain C. violaceum CV026 [24,25]. The 16S rDNA based identification of specifically selected isolate for its high AHL degradation ability was performed as described previously [28]. Approximately 1,500 bp of the 16S rDNA was amplified using the universal eubacterial primers 27F (5′-GAGAGTTTGATCCTGGCTCAG) and 1492R (5′-TACCTTGTTACGACTT). The 16S rRNA gene sequence information of amplified fragment was obtained through the commercial sequencing services of Eurofins MWG GmbH, Ebersberg, Germany. The sequence data was matched using the tools provided at NCBI database (http://www.ncbi.nlm.nih.gov). The phylogenetic analysis was performed with this partial sequence using the default parameters provided at RDP database (http://rdp.cme.msu.edu). The branching pattern was analysed using 100 bootstrap replicates.

Preparation of natural AHL extracts

Natural AHL was extracted according to Shaw et al. [29] from the culture supernatant of late exponential growth phase Pcc BR1culture. Cell-free supernatant was prepared by centrifugation at 14,000 ×g for 10 min at 4°C. AHL molecules were extracted thrice with equal volume of cold acidified ethyl acetate (0.01% v/v acetic acid) and concentrated using rotary evaporation. The concentrated AHL extract was dissolved in acidified ethyl acetate or acetonitrile and stored at -20°C. All synthetic AHL stock solutions were prepared at 1 mg mL^-1 concentration in acidified ethyl acetate and stored at -20°C.

AHL bioassays

For estimation of extracted AHL or that present in culture supernatant, violacein broth bioassay was used [30]. The purple color of C. violaceum CV026 pigment produced in response to the additions of spent medium extract or synthetic autoinducer was expressed as violacein units = (Abs_585nm / Abs_660nm)×1000. To determine the concentration of AHL molecules, induction of bioluminescence in E. coli plasmid-borne lux sensors (pSB401 and pSB1075) was analyzed using Adavace Celsis Luminometer (Celsis, UK) and Glomax-Multi⁺Microplate Luminometer (Promega, UK). Microtitre plate assay for assessment of AHL activation of E. coli lux biosensor cells carrying reporter plasmids was performed as follows: Aliquots of the AHL being assayed were made in acetonitrile (HPLC grade, Sigma-Aldrich, UK) and dried overnight. 200μl of diluted E. coli lux biosensor cells were added to the dried AHL samples and the assay plates were incubated at 30°C, on a rocking platform for 4 h, and bioluminescence was measured using microplate Luminometer [31].

Enzyme assays

Polygalacturonase (PG) and Pectate lyase (PL) activities were determined as described earlier [26,27]. AHL degrading activity was determined by measuring residual AHL concentration using bioluminescence based detection system. The purified protein solutions were mixed with 1:5 volumes of 125 μM AHL in 10 mM Phosphate Buffer Saline (PBS; pH 6.5) incubated at 30°C for 2 h. Residual AHL concentration was detected using E. coli lux (pSB401 or pSB1075) biosensors cells in Luminometer as mentioned in AHL bioassay as well as by HPLC. One unit of AHL degrading activity was defined as the amount of enzyme required to degrade 1 nM AHL molecules at 30°C and pH 6.5 per minute.

Co-cultivation of Pcc BR1 and AHL degrading isolates

For co-cultivation experiments, Pcc BR1 and the selected AHL degrading isolates were grown overnight separately in NB at 30°C. PccBR1 was inoculated in NBP at 1.75 ×10⁶ CFU mL^-1 along with AHL degrading isolates at 1.12–4.5 ×10⁸ CFU mL^-1. All the co-culture combinations were performed in triplicate with incubation at 30°C under 140 rpm shaking condition. To analyze the growth rate, bacterial cells were enumerated as CFU by plating serially diluted samples in triplicate on Violet Red Bile Agar (VRBA) medium (HiMedia Laboratories Pvt. Ltd., India) and NB agar. The CFU counts of the two cultures could be separately recorded due to difference in colony morphology on the selective media [32]. Samples were withdrawn at 6 h for determination of AHL amounts in culture supernatant using AHL broth bioassay, while PG and PL activities were determined from culture supernatant as mentioned earlier.

Purification and characterization of AHL degrading enzyme

Overnight grown cells of Delftia sp. VM4 were harvested from the culture broth by centrifugation at 12,000 rpm for 10 min at 4°C. Cell pellet was resuspended in 10 mM Tris-HCl (pH 6.5) containing 0.1 mM PMSF, and then homogenized using a cell sonicator. The precipitated proteins were collected from a 40–70% saturation of ammonium sulfate, dissolved in 10 mM Tris-HCl (pH 6.5), and dialyzed against the same buffer. Partial purification of the AHL-degrading enzymes was performed using anionic-exchange column chromatography (DEAE-sepharose CL-6B column, 0.8×12 cm) equilibrated with the 10 mM Tris-HCl (pH 6.5). The dialysate was applied to a column and eluted with NaCl gradient (0–1.0 M). The active fractions from DEAE-sepharose CL-6B column showing AHL-degrading enzyme activity were collected, concentrated by centrifugal concentrator tubes (Centriprep-10, Millipore), and loaded onto a Sephadex G50-80 column (1.5×50 cm) equilibrated with the 10 mM Tris-HCl (pH 6.5). Active fractions were pooled, concentrated and then mixed with sample buffer for native gel electrophoresis and resolved on 12% SDS-PAGE in duplicate as described by Park et al. [22]. Following electrophoresis, one of the gel was stained with silver salts [33] and the other was renatured by washing with 10 mM Tris-HCl (pH 6.5) containing 2.0% Triton X-100 for 15 min with gentle shaking at room temperature. The renatured gel was washed twice with 10 mM Tris-HCl (pH 6.5) then bands were sliced with reference to protein bands on the silver stained gel. The gel slices were mixed with 20 μM HHL, and incubated with gentle shaking at 30°C for 2 h. After the incubation, the reaction mixture was filtered through 0.45 μm nitrocellulose membrane filter and 30 μl filtrate was added to 170 μl of C. violaceum CV026 in TY broth medium (OD 600nm, 0.002) into a microtitre plate. Peptide mass fingerprinting analysis of the active protein band possessing AHL degrading activity was carried out at The Centre for Genomic Application (TCGA), New Delhi, India. The protein samples were processed for alkylation, reduction and tryptic digestion, the mass finger printing was done by MALDI-TOF MS and was also subjected to MS/MS analysis by LC-MS. Multiple amino acid sequence alignment of purified protein sample was carried out. Sequence alignment was performed with ClustalW (http://www.ddbj.nig.ac.jp/search/clustalw-j.html) and presented with Boxshade 3.21 (http://www.ch.embnet.org/software/BOXform.html). Prediction of putative secondary structure was done using a web-based service NetSurfP (http://www.cbs.dtu.dk/services/NetSurfP).

Effect of temperature and pH on enzyme activity and kinetics

The temperature optima were determined by incubating an appropriate amount of enzyme with 125 μM HHL in 50 mM Phosphate buffer (pH 6.5) at various temperatures ranging from 20–70°C for 2h. The effect of pH on enzyme activity was determined by measuring activity at 30°C for 2 h, using different buffers: 50 mM Na-acetate buffer (pH 3.5, 4.15, 4.55, 5.0, 5.5, 6.0), 50 mM phosphate buffer (pH 6.0,6.2, 6.4, 6.6, 6.8, 7.0) and 50 mM Tris-HCl buffer (pH 6.6, 6.74, 7.0, 7.2, 7.4, 7.6). AHL bioassay was performed as mentioned above in section. The kinetic analysis of AHL degrading enzyme was performed according to Maisuria and Nerurkar [27].

HPLC, EI-MS and NMR analyses of AHLs and its degradation product

AHL degradation products of purified AHL degrading enzyme reaction and AHLs were analyzed using reverse-phase HPLC, NMR and electron impact ionization-mass spectrometry (EI-MS) [11,22]. For the reverse-phase HPLC analysis, the sample was dissolved in 0.1 mL 50% (v/v) methanol (in MiliQ water) and analyzed using a C₁₈ reverse-phase column (250×4.6mm S-4μm, 80A, YMC co. ltd. JH08S04-2546WT). The fractions were separated by isocratic gradient (1–100%) of water: formic acid (0.1%, pump A) and acetonitrile: formic acid (0.1%, pump B) at a flow rate of 0.3 mL min^-1 detected by SPD-10UV detector at 210nm in HPLC unit (Shimadzu Corp., Japan). The EI-MS was performed using a DSQII GC/MS with TRACE GC Ultra unit (Thermo scientific), and high resolution ¹H and ¹³C NMR was performed using Bruker NMR spectrometer. For MS analysis the sample was dissolved in 50% (v/v) methanol (in MiliQ water) and ionized by a positive-ion electron impact, while for NMR samples was dissolved in D₂O. As a control standard, 5 mM of synthetic HHL and HSL (Sigma-Aldrich, India) dissolved in 50% (v/v) methanol (in MiliQ water) were used as AHL standards while the uninoculated medium was used as an additional control. The percentage of AHL degradation was determined by estimation of AHL with respect to known concentration that was loaded.

Results and Discussion

Isolated Delftia sp. VM4 can inactivate different types of AHLs

Isolate VM4 showed consistently high AHL degrading activity in all AHL bioassay based screening and was identified as Delftia sp. based on its 16S rDNA partial sequence. Approximately 1000 nucleotide sequence of 16S rRNA gene (GenBank accession number JF717756) showed 100% identity to Delftia tsuruhatensis on BLAST search at NCBI database. This isolate VM4, on phylogentic analysis using 16S rRNA gene sequences, clustered along with Delftia tsuruhatensis, Delftia lacustris and Delftia acidovorans strains (S1 Fig), therefore, henceforth designated as Delftia sp. VM4. An AHL-degrading soil isolate belonging to genus Delftia previously reported by Jafra et al. [21] was found to inactivate exogenous AHLs supplemented in growth medium and attenuate the maceration of potato tissue caused by P. carotovorum. This inactivating mechanism was attributed to an unknown factor involved in AHL degradation. The purification and detailed investigation of AHL degradation activity from Delftia sp. has not been reported hitherto, which we aimed to do in this study. The effect of Delftia sp. VM4 on the growth, AHL accumulation and virulence determinant enzyme production of Pcc BR1 when analyzed in the co-culture condition showed that the PL production of Pcc BR1 was significantly inhibited by Delftia sp. VM4 without affecting its growth (Fig 1A). It reduced the OHHL accumulation significantly which resulted in suppression of virulence determinant enzymes (PG and PL) production of Pcc BR1 during co-culture (Fig 1A and 1B). The pH of the medium monitored intermittently was seen to increase slightly above neutrality. At 6 h of growth, the medium pH increased from 7.2 to 7.8, might not be responsible for decline in OHHL production as its accumulation was inhibited within 6 h during co-culture (Fig 1B). Delftia sp. VM4 interferes with quorum-sensing signaling of Pcc BR1 when they live commensally in vitro, and such signal interruption causes drastic attenuation of Pcc BR1 virulence determinants. Furthermore, Delftia sp. VM4 did not produce an antibiotic-like material to hinder the growth of Pcc BR1. This result supports the possibility of involvement of quorum quenching factor in AHL degradation by this selected isolate Delftia sp. VM4 preventing virulence of Pcc BR1.

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Fig 1. Effect of Delftia sp. VM4 on growth, AHL accumulation and virulence determinants production by PccBR1.

[A] Time course of bacterial growth (circles, triangles) and PL production (squares). Pcc BR1 and Delftia sp. VM4 were incubated and grown separately (open symbols) or co-cultured (solid symbols). [B] AHL accumulation (diamonds), PG production (stars) and pH changes (triangles) in culture supernatant during co-culture of Pcc BR1 and Delftia sp. VM4. PccBR1 was inoculated alone (open symbols) or co-cultured (solid symbols). The experiment was repeated n = 3 times. The results are means ± SD (not shown where smaller than symbols).

https://doi.org/10.1371/journal.pone.0138034.g001

The residual amount of HHL or OOHL in the culture medium significantly decreased with increase in cell density when the growth of Delftia sp. VM4 was measured with HHL and OOHL individually as the sole source of carbon and energy. Most of the HHL and OOHL in the culture medium were consumed after 10 h of cultivation (S2A Fig). No growth was observed in minimal medium without AHL. It has been reported that the half-life of AHL is less than 3 h under alkaline conditions (pH >8.0) [19]. During 10 h of cultivation in monoculture of Delftia sp. VM4, the pH of the culture medium increased to 6.7 (S2A Fig). Thus, the dramatic decrease of AHLs in the medium was unlikely to have been due to action of pH. Clearly, Delftia sp. VM4 was able to utilize HHL and OOHL as both the sole carbon and nitrogen source. This strain effectively degraded different types of AHL molecules (HHL, OHHL, OOHL and DHL) based on biosensor assay (S2B Fig). In particular, OHHL was degraded efficiently within 60 min, while DHL degradation was poor as even after 120 min the degradation was incomplete. The quorum quenching activity of Delftia sp. VM4 was lost after treatment with trypsin and proteinase K (S2B Fig). These observations suggest that the AHL degradation of Delftia sp. VM4 could be enzymatic reaction. Similar characteristics of AHL degrading activity of other Gram-negative bacterial strains such as V. paradoxus VAI-C and P. aeruginosa PAO1 in minimal medium supplemented with AHLs as sole energy source have been reported by other groups [19,34].

Purification and characterization of AHL degrading enzyme of Delftia sp. VM4

The AHL degrading activity of Delftia sp.VM4 was observed only in cell lysates, indicating that the enzyme is an intracellular protein. Extracellular activity in the supernatant was absent, thus the AHL-degrading enzyme was purified from the cell lysate using DEAE- sepharose CL6B and Sephadex G50-80 column chromatography. The final step of purification on Sephadex G50-80 column chromatography gave 155.8 fold purification and 12% yield of the enzyme (S1 Table). The active fractions with HHL degrading activity eluted from column chromatography were concentrated and separated on 12% SDS-PAGE. The purified fraction from gel permeation step gave a single band of 82 kDa size (Fig 2A). The enzyme activity assay of individual intense protein bands separated on SDS-PAGE was carried out with renatured gel slices (Fig 2B). The single protein band of about 82 kDa size (marked as band-iii, Fig 2A) exhibited AHL degrading activity as shown in corresponding wells of microtitre plate (Fig 2B). Bands labeled as i, ii, iv, v and vi of elute from DEAE-sepharose column were also tested in microtitre well plate assay shown in corresponding wells. Only protein band-iii of 82 kDa size possessed AHL degrading activity and was identified as AHL acylase type since it produced the product characteristic of HHL cleavage which was identified as HSL in HPLC (Fig 2C and 2D) and EI-MS (Fig 2E). The EI-MS analysis of the product revealed abundance of HSL with M+H ion at m/z 99.05 signifying that the cleavage of HHL (total M+H ion m/z 199 and identity M+H ion m/z 143) by the action of AHL degrading enzyme produced two products viz. HSL and acyl-moiety. Moreover, HSL structural information of four carbon ring structure was validated using ¹³C and ¹H NMR as shown in S3A and S3B Fig. In order to identify the protein, fragments of tryptic digest of the protein eluted from the band (82 kDa on SDS-PAGE) were subjected to MALDI-TOF and MS/MS analyses. The spectrum obtained was matched with proteins in the NCBI GenBank database, using the Mascot search program. The search yielded a top score of 83.6 for hypothetical protein Daci_4366 [protein scores greater than 67 are significant at P<0.05 according to Xu et al. [35]]. Furthermore, the selected tryptic peptides of 901.39 m/z, 1008.46 m/z and 1630.84 m/z sequenced by MS revealed an amino acid sequence of GEWNLQR, DIQPMLHR and TTQLQNTEPLLAFR respectively, corresponding to residues of hypothetical protein Daci_4366 (gi| 5749954) of Delftia acidovorans SPH-1 (S4 Fig). The selected tryptic peptide of 1630.84 m/z further sequenced by MS/MS revealed the identical amino acid sequence as above, and the Mascot search yielded a top protein score of 177 for the hypothetical protein Daci_4366.

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Fig 2. Characterization of AHL-acylase of Delftia sp. VM4.

(A) Silver stained 12% SDS-PAGE during purification of AHL acylase from Delftia sp. VM4. Lane: M, PMWH- protein marker; 1, ammonium sulphate precipitates; 2, eluate from DEAE-sepharose column; 3, eluate from Sephadex column. (B) Microtitre plate assay for the AHL degrading activity with 0.05 mM HHL of the renatured gel slices (bands i-vi) from the SDS-PAGE was assayed using C. violaceum CV026 biosensor based bioassay. HHL (0.05 mM) was loaded as the control (well-c). HPLC profile of (C) HHL without purified AHL acylase, (D) HHL degradation by purified AHL acylase, (E) EI-MS analysis of degraded product (HSL) from HHL.

https://doi.org/10.1371/journal.pone.0138034.g002

Interestingly, up till now the AHL acylases from Streptomyces sp. M664 [36], Ralstonia sp. XJ12B [20], Shewanella sp. MIB015 [37] and Anabaena sp. PCC7120 [38], PvdQ [34] and QuiP [39] from P. aeruginosa, HacA and HacB from P. syringae [40] were reported to contain domains characteristic of the Ntn-hydrolase (Ntn: N-terminal nucleophile) superfamily which are described as those possessing a wide variety of hydrolytic activities and distinct folds. In contrast, the purified enzyme (82 kDa) of Delftia sp. VM4 under study here possessed similarity with an α/β-hydrolase fold protein. This type of AHL acylase owning α/β-hydrolase fold was already identified in Ochrobactrum sp. A44 [41], which belonged to the carboxylic ester hydrolases (EC 3.1.1) as it possesses a nucleophile-His-Acid catalytic triad characteristic of typical carboxylic ester hydrolase [42]. An α/β-hydrolase fold protein was first time described by Ollis et al. [43]. The 3D-Jury structure based analysis using BioinfoMetaServer revealed that the hypothetical protein Daci_4366 has structural similarity with different Ntn-hydrolases. Inspection of genome database of Delftia acidovorans SPH-1, revealed α/β-hydrolase fold homologue (Daci_1886) that was also similar to reported α/β-hydrolase type AHL acylase of Ochrobactrum sp. A44 [41] and other homologues. The similarity of hypo_Daci4366 to the carboxylic ester hydrolases implies the presence of a catalytic triad composed of His, Asp or Glu and Ser (Fig 3). In summing up, it can be said that the purified enzyme from Delftia sp. VM4 represents a novel class of AHL-inactivating acylases. The constructed structure of α/β-hydrolase fold protein of Delftia predicted using online tool Easypred and processed with PymoL software is shown in Fig 4.

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Fig 3. Multiple amino acid sequence alignment for AHL-acylase of Delftia sp. VM4 with AHL-acylases and α/β-hydrolases.

In addition to hypothetical protein (Daci_4366, indicated as hypo_Daci4366, gi| 5749954) from Delftia acidovorans, acylase homologues used in the alignment includes, Ochrobactrum anthropi ATCC 49188 (AHLacylase_Oanthropi, gi|151560055), Ochrobactrum sp. A44 (AHL acylase_OchroA44, gi|298370747), and α/β hydrolase fold homologues includes, Nitrobacter winogradskyi Nb-255 (AbH-N.winogr, gi| 74419560), Rhodococcus erythropolis SK121 (AbH-Rerythro, gi|229491600), Parvibaculum lavamentivorans DS-1 (AbH_Plavament, gi|154156608) and D. acidovorans SPH-1 (Daci_1886, gi| 5747443, indicated as abHf_Daci1886). White letters on a black background depict identical residues, and similar residues are depicted by black letters on a gray background and gaps are represented by dashes to facilitate alignment. Conserved residues of relevance to autoproteolysis and catalysis in known acylases are marked with asterisks. ‘*’, identical residue; ‘dot’, semi-conserved substitution; ‘arrow’, putative β strands subunits; ‘tube shape’, putative α helices subunits. Residues positions known or thought to be possessed conservative amino acid residues (S, H, D/E) typical for the catalytic site of an α/β hydrolase are marked by triangles.

https://doi.org/10.1371/journal.pone.0138034.g003

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Fig 4. Predicted structural model of α/β-hydrolase fold protein of Delftia.

Residue positions of known or deduced conserved amino acid residues (S, H, D/E) typical for the catalytic site of an α/β hydrolase are marked in blue, red and green respectively.

https://doi.org/10.1371/journal.pone.0138034.g004

Biochemical properties of purified AHL acylase from Delftia sp. VM4

Purified AHL acylase activity was examined with HHL as substrate. The optimal pH for the AHL-degrading activity of purified AHL acylase was examined using HHL as substrate at 30°C. The AHL acylase activity was enhanced with increasing pH (above pH 5) and reached maximum at pH 6.2 (Fig 5A). At above pH 7.5, HHL is possibly inactivated by alkylation [44]. There is sparse information available in literature for optimum temperature and pH of AHL acylase. In contrast to this, AHL lactonase (AiiM) from Microbacterium testaceum StLB037 is reported as showing maximum activity at 37°C and pH 8.0 [45]. The activity of purified AHL acylase was (optimum more than 80%) at temperature range of 20–40°C, which was drastically decreased at elevated temperatures 50 and 60°C (Fig 5B). The kinetic and thermodynamic parameters for hydrolysis of AHL substrates (OHHL, OOHL and HHL) by purified AHL acylase from Delftia sp. VM4 were determined (Table 1) as described in our previous study [27]. This AHL acylase showed higher affinity with OHHL and OOHL compared to HHL as adjudged from lower K_m values. Also thermodynamic parameters for substrate hydrolysis of purified AHL acylase supported this observation (Table 1). There is no information available regarding kinetic and thermodynamic parameters of any AHL acylase in literature. However, these kinetic properties have been described by Wang et al. [46] for AHL-lactonase (AiiA) of Bacillus sp. In comparison to this AHL-lactonase (AiiA), the purified AHL acylase of Delftia sp. VM4 was found to be catalytically efficient for HHL, OHHL and OOHL hydrolyses. The K_m, k_cat and k_cat/K_m values of AHL-lactonase (AiiA) were reported as 3.83 mM, 35.67 sec^-1, 9.31 mM^-1 sec^-1 respectively for HHL hydrolysis; and 2.28 mM, 22.17 sec^-1, 9.72 mM^-1 sec^-1 respectively for OOHL hydrolysis. If the k_cat/K_m values for the hydrolysis of HHL, OHHL and OOHL substrates by the purified AHL acylase of Delftia sp. VM4 (Table 1) is compared with k_cat/K_m values of other wild-type lactonase homologues (VmoLac, GKL and MCP) [47–49], the purified AHL acylase ranks the highest. This can be attributed to elevated catalytic efficiency (k_cat/K_m) with effective substrate affinity (lower K_m values) and turnover number (higher k_cat values) (Table 1). The cations including Na⁺, K⁺, Mg²⁺ and Ca²⁺, showed no effect on AHL acylase activity at 2 mM concentration (Table 2). On the other hand, purified AHL acylase was partially inhibited by Ba²⁺, Mn²⁺, Ni²⁺ and Zn²⁺ at 1 mM and completely inhibited by Cd²⁺, Cu²⁺ and Pb²⁺ (Table 2).

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Fig 5. Effect of (A) pH and (B) temperature on purified AHL-acylase actvity of Delftia sp. VM4.

https://doi.org/10.1371/journal.pone.0138034.g005

Download:

Table 1. Michaelis-Menten constants and thermodynamic parameters for substrate hydrolysis of purified AHL acylase from Delftia sp. VM4.

https://doi.org/10.1371/journal.pone.0138034.t001

Download:

Table 2. Effect of cations on purified AHL acylase activity of Delftia sp. VM4.

https://doi.org/10.1371/journal.pone.0138034.t002

In summary, the present study deals with an efficient AHL degrading soil bacterium Delftia sp. VM4 capable of hydrolyzing different types of AHLs and by virtue of this enzyme it could afford protection against the virulence of Pcc BR1. The present study supported that the efficacy of Delftia sp. VM4 in preventing the production of virulence determinants in Pcc BR1 depends upon its ability to produce AHL acylase that inactivates AHL molecules by hydrolyzing amide bond between lactone ring and fatty acyl moiety. To the best of our knowledge, this AHL acylase belonged to a novel α/β hydrolase family fold, while all previously known AHL acylases belonged to amidase family. Further research on such AHL acylase would help in developing a new class of potential anti-virulence approach with broad spectrum activity. The AHL acylase from Delftia sp. VM4 showed efficient biochemical properties for the degradation of AHL signal and thus can be a useful anti-virulence agent against many bacterial pathogens where virulence is regulated by AHL type quorum sensing. Moreover, analysis on the application of this novel AHL acylase against many pathogens in vivo or in situ should not be ignored.

Supporting Information

S1 Fig. Phylogenetic analysis of isolate VM4 within the genus Delftia.

The branching pattern was generated using Weighbor joining method. The Genbank accession number of the 16S rDNA sequences are indicated in parenthesis. The number at each branch indicates the bootstrap values out of 100. Delftia litopenaei is used as out-group.

https://doi.org/10.1371/journal.pone.0138034.s001

(TIF)

S2 Fig. [A] Time course of growth of Delftia sp. VM4 in minimal medium containing HHL or OOHL as a sole carbon source. Culture growth in the medium with HHL (filled circle), OOHL (filled triangle) and without AHL (open square) was assessed based on the O.D. at 600nm. The residual HHL (open circle) and OOHL (open triangle) concentration in the culture was measured using a bioassay. The pH change in culture supernatant was monitored during growth curve. [B] Inactivation of various AHLs by a cell suspension of Delftia sp. VM4.

Equal volumes of a diluted cell suspension (OD600nm 1.0) and a PBS (pH-6.5) containing 100 μM of each synthetic AHLs (HHL, OOHL, DHL) and OHHL (extracted AHLs produced by Pcc BR1) were mixed and incubated at 30°C. For the experimental control a culture suspension was first treated with trypsin and proteinase K, and then mixed with 100 μM OOHL. Experiments were repeated n = 3 times; the results shown are means ± S.D.

https://doi.org/10.1371/journal.pone.0138034.s002

(TIF)

S3 Fig. (A) Proton-decoupled ¹³C-NMR spectrum of HHL degraded product (i.e. HSL) by purified AHL acylase of Delftia sp. VM4. The spectrum was measured in D₂O at 100 MHz (chemical shifts in p.p.m. from tetramethylsilane) and the following data were obtained: 174.42 C-1, 67.24 C-4, 48.43 C-2, and 26.63 C-3. (B) High-resolution ¹H-NMR spectrum HHL degraded products (i.e. HSL) by purified AHL acylase of Delftia sp. VM4.

The spectrum was measured in D₂O at 400 MHz (chemical shifts in p.p.m. from tetramethylsilane) and the data compared with standard HSL spectrum, indicate identical values.

https://doi.org/10.1371/journal.pone.0138034.s003

(TIF)

S4 Fig. Tryptic digest fingerprint and sequence matches after MALDI-TOF MS/MS analysis of purified AHL acylase from Delftia sp. VM4.

https://doi.org/10.1371/journal.pone.0138034.s004

(TIF)

S1 Table. Purification of AHL acylase from Delftia sp. VM4.

https://doi.org/10.1371/journal.pone.0138034.s005

(PDF)

Acknowledgments

Authors would like to thank Chris Michiels (Katholieke Universiteit Leuven, Belgium) for providing AHL biosensor Chromobacterium violaceum CV026 and also thankful to Timothy Aldsworth (University of Hertfordshire, UK) and Matthew Fletcher (University of Nottingham, UK) for providing Lux-based AHL biosensor strain. Sincere thanks to Arun K. Chatterjee and Yaya Cui (University of Missouri, USA) for providing Las-based biosensor strain. Special thanks to Shailesh R. Shah, Ashutosh V. Bedekar, Harish Talele and Harnish H. Soni (Department of Chemistry, The M. S. University of Baroda, India) for timely help in NMR and MS analysis.

Author Contributions

Conceived and designed the experiments: VBM ASN. Performed the experiments: VBM. Analyzed the data: VBM. Contributed reagents/materials/analysis tools: VBM ASN. Wrote the paper: VBM ASN.

References

1. Rutherford ST, Bassler BL. Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med. 2012.
- View Article
- Google Scholar
2. Barnard AML, Salmond GPC. Quorum sensing in Erwinia species. Anal Bioanal Chem. 2007; 387: 415–423. pmid:16943991
- View Article
- PubMed/NCBI
- Google Scholar
3. Fuqua WC, Winans SC, Greenberg EP. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J Bacteriol. 1994: 176: 269–275. pmid:8288518
- View Article
- PubMed/NCBI
- Google Scholar
4. Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol. 2001; 55: 165–199. pmid:11544353
- View Article
- PubMed/NCBI
- Google Scholar
5. Von Bodman SB, Bauer WD, Coplin DL. Quorum sensing in plant-pathogenic bacteria. Annu Rev Phytopathol. 2003; 41: 455–482. pmid:12730390
- View Article
- PubMed/NCBI
- Google Scholar
6. Barnard AML, Bowden SD, Burr T, Coulthurst SJ, Monson RE, Salmond GPC. Quorum sensing, virulence and secondary metabolite production in plant soft-rotting bacteria. Philos Trans R Soc Lond B Biol Sci. 2007; 362: 1165–1183. pmid:17360277
- View Article
- PubMed/NCBI
- Google Scholar
7. Burr T, Barnard AML, Corbett MJ, Pemberton CL, Simpson NJL, Salmond GPC. Identification of the central quorum sensing regulator of virulence in the enteric phytopathogen, Erwinia carotovora: the VirR repressor. Mol Microbiol. 2006; 59: 113–125. pmid:16359322
- View Article
- PubMed/NCBI
- Google Scholar
8. Dong Y-H, Zhang L-H. Quorum sensing and quorum-quenching enzymes. J Microbiol. 2005; 43: 101–109. pmid:15765063
- View Article
- PubMed/NCBI
- Google Scholar
9. Dong Y, Zhang X-F, Xu J, Zhang L. Insecticidal Bacillus thuringiensis silences Erwinia carotovora virulence by a new form of microbial antagonism, signal interference. Appl Environ Microbiol. 2004; 70: 954–960. pmid:14766576
- View Article
- PubMed/NCBI
- Google Scholar
10. Pirhonen M, Flego D, Heikinheimo R, Palva ET. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO J. 1993; 12: 2467–2476. pmid:8508772
- View Article
- PubMed/NCBI
- Google Scholar
11. Bainton NJ, Stead P, Chhabra SR, Bycroft BW, Salmond GP, Stewart GS, et al. N-(3-oxohexanoyl)-L-homoserine lactone regulates carbapenem antibiotic production in Erwinia carotovora. Biochem J. 1992; 288: 997–1004. pmid:1335238
- View Article
- PubMed/NCBI
- Google Scholar
12. Bauer WD, Robinson JB. Disruption of bacterial quorum sensing by other organisms. Curr Opin Biotechnol. 2002; 13: 234–237. pmid:12180098
- View Article
- PubMed/NCBI
- Google Scholar
13. Dong YH, Wang LH, Xu JL, Zhang HB, Zhang XF, Zhang LH. Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase. Nature 2001; 411: 813–817. pmid:11459062
- View Article
- PubMed/NCBI
- Google Scholar
14. Kalia VC. Quorum sensing inhibitors: an overview. Biotechnol Adv. 2013; 31: 224–245. pmid:23142623
- View Article
- PubMed/NCBI
- Google Scholar
15. Czajkowski R, Jafra S. Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules. Acta Biochim Pol. 2009; 56: 1–16. pmid:19287806
- View Article
- PubMed/NCBI
- Google Scholar
16. Lee SJ, Park S, Lee J-J, Yum D, Koo B, Lee J-K. Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis. Appl Environ Microbiol. 2002; 68: 3919–3924. pmid:12147491
- View Article
- PubMed/NCBI
- Google Scholar
17. Dong YH, Xu JL, Li XZ, Zhang LH. AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A. 2000; 97: 3526–3531. pmid:10716724
- View Article
- PubMed/NCBI
- Google Scholar
18. d’Angelo-Picard C, Faure D, Penot I, Dessaux Y. Diversity of N-acyl homoserine lactone-producing and-degrading bacteria in soil and tobacco rhizosphere. Environ Microbiol. 2005; 7: 1796–1808. pmid:16232294
- View Article
- PubMed/NCBI
- Google Scholar
19. Leadbetter JR, Greenberg EP. Metabolism of acyl-homoserine lactone quorum-sensing signals by Variovorax paradoxus. J Bacteriol. 2000; 182: 6921–6926. pmid:11092851
- View Article
- PubMed/NCBI
- Google Scholar
20. Lin Y-H, Xu J-L, Hu J, Wang L-H, Ong SL, Leadbetter JR, et al. Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes. Mol Microbiol. 2003; 47: 849–860. pmid:12535081
- View Article
- PubMed/NCBI
- Google Scholar
21. Jafra S, Przysowa J, Czajkowski R, Michta a, Garbeva P, van der Wolf JM. Detection and characterization of bacteria from the potato rhizosphere degrading N-acyl-homoserine lactone. Can J Microbiol. 2006; 52: 1006–1015. pmid:17110970
- View Article
- PubMed/NCBI
- Google Scholar
22. Park S-Y, Lee SJ, Oh T-K, Oh J-W, Koo B-T, Yum D-Y, et al. AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria. Microbiology 2003; 149: 1541–1550. pmid:12777494
- View Article
- PubMed/NCBI
- Google Scholar
23. Jones S, Yu B, Bainton NJ, Birdsall M, Bycroft BW, Chhabra SR, et al. The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J. 1993; 12: 2477–2482. pmid:8508773
- View Article
- PubMed/NCBI
- Google Scholar
24. McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Camara M, et al. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 1997; 143: 3703–3711. pmid:9421896
- View Article
- PubMed/NCBI
- Google Scholar
25. Steindler L, Venturi V. Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors. FEMS Microbiol Lett. 2007; 266: 1–9. pmid:17233715
- View Article
- PubMed/NCBI
- Google Scholar
26. Maisuria VB, Patel VA, Nerurkar AS. Biochemical and thermal stabilization parameters of polygalacturonase from Erwinia carotovora subsp. carotovora BR1. J Microbiol Biotechnol. 2010; 20: 1077–1085. pmid:20668400
- View Article
- PubMed/NCBI
- Google Scholar
27. Maisuria VB, Nerurkar AS. Biochemical properties and thermal behavior of pectate lyase produced by Pectobacterium carotovorum subsp. carotovorum BR1 with industrial potentials. Biochem Eng J. 2012; 63: 22–30.
- View Article
- Google Scholar
28. Maisuria VB, Nerurkar AS. Characterization and differentiation of soft rot causing Pectobacterium carotovorum of Indian origin. Eur J Plant Pathol. 2012; 136: 87–102.
- View Article
- Google Scholar
29. Shaw PD, Ping G, Daly SL, Cha C, Cronan JE, Rinehart KL, et al. Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc Natl Acad Sci U S A. 1997; 94: 6036–6041. pmid:9177164
- View Article
- PubMed/NCBI
- Google Scholar
30. Blosser RS, Gray KM. Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers. J Microbiol Methods 2000; 40: 47–55. pmid:10739342
- View Article
- PubMed/NCBI
- Google Scholar
31. Winson MK, Swift S, Fish L, Throup JP, Jørgensen F, Chhabra SR, et al. Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiol Lett. 1998; 163: 185–192. pmid:9673021
- View Article
- PubMed/NCBI
- Google Scholar
32. Liao C-H, Shollenberger LM. Enumeration, resuscitation, and infectivity of the sublethally injured erwinia cells induced by mild Acid treatment. Phytopathol. 2004; 94: 76–81.
- View Article
- Google Scholar
33. Sambrook J, Green MR. Molecular cloning- A laboratory manual. 4th ed. Inglis J, Boyle A, Gann A, editors. New York: Cold spring harbour laboratory press. 2012.
34. Huang JJ, Han J, Zhang L, Leadbetter JR. Utilization of acyl-homoserine lactone quorum signals for growth by a soil Pseudomonad and Pseudomonas aeruginosa PAO1. Appl Environ Microbiol. 2003; 69: 5941–5949. pmid:14532048
- View Article
- PubMed/NCBI
- Google Scholar
35. Xu S, Zhao L, Larsson A, Smeds E, Kusche-Gullberg M, Venge P. Purification of a 75 kDa protein from the organelle matrix of human neutrophils and identification as N-acetylglucosamine-6-sulphatase. Biochem J. 2005; 387: 841–847. pmid:15595925
- View Article
- PubMed/NCBI
- Google Scholar
36. Park S, Kang H, Jang H, Lee J, Koo B, Yum D-Y. Identification of extracellular N-acylhomoserine lactone acylase from a Streptomyces sp. and its application to quorum quenching. Appl Environ Microbiol. 2005; 71: 2632–2641. pmid:15870355
- View Article
- PubMed/NCBI
- Google Scholar
37. Morohoshi T, Nakazawa S, Ebata A, Kato N, Ikeda T. Identification and characterization of N-acylhomoserine lactone-acylase from the fish intestinal Shewanella sp. strain MIB015. Biosci Biotechnol Biochem. 2008; 72: 1887–1893. pmid:18603799
- View Article
- PubMed/NCBI
- Google Scholar
38. Romero M, Diggle SP, Heeb S, Cámara M, Otero A. Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase. FEMS Microbiol Lett. 2008; 280: 73–80. pmid:18194337
- View Article
- PubMed/NCBI
- Google Scholar
39. Huang JJ, Petersen A, Whiteley M, Leadbetter JR. Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosaPAO1. Appl Environ Microbiol. 2006; 72: 1190–1197. pmid:16461666
- View Article
- PubMed/NCBI
- Google Scholar
40. Shepherd RW, Lindow SE. Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology. Appl Environ Microbiol. 2009; 75: 45–53. pmid:18997027
- View Article
- PubMed/NCBI
- Google Scholar
41. Czajkowski R, Krzyżanowska D, Karczewska J, Atkinson S, Przysowa J, Lojkowska E, et al. Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase. Environ Microbiol Rep. 2011; 3: 59–68. pmid:23761232
- View Article
- PubMed/NCBI
- Google Scholar
42. Holmquist M. Alpha/Beta-hydrolase fold enzymes: structures, functions and mechanisms. Curr Protein Pept Sci. 2000; 1: 209–235. pmid:12369917
- View Article
- PubMed/NCBI
- Google Scholar
43. Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, et al. The alpha/beta hydrolase fold. Protein Eng. 1992; 5: 197–211. pmid:1409539
- View Article
- PubMed/NCBI
- Google Scholar
44. Yates EA, Philipp B, Buckley C, Atkinson S, Chhabra SR, Sockett RE, et al. N-acylhomoserine lactones undergo lactonolysis in a pH-, temperature-, and acyl chain length-dependent manner during growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa. Infect Immun. 2002; 70: 5635–5646. pmid:12228292
- View Article
- PubMed/NCBI
- Google Scholar
45. Wang W-Z, Morohoshi T, Ikenoya M, Someya N, Ikeda T. AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum. Appl Environ Microbiol. 2010; 76: 2524–2530. pmid:20173075
- View Article
- PubMed/NCBI
- Google Scholar
46. Wang L-H, Weng L-X, Dong Y-H, Zhang L-H. Specificity and enzyme kinetics of the quorum-quenching N-Acyl homoserine lactone lactonase (AHL-lactonase). J Biol Chem. 2004; 279: 13645–13651. pmid:14734559
- View Article
- PubMed/NCBI
- Google Scholar
47. Chow JY, Wu L, Wen SY. Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp. paratuberculosis K-10 in the amidohydrolase superfamily. Biochem. 2009; 48: 4344–4353.
- View Article
- Google Scholar
48. Chow JY, Xue B, Lee KH, Tung A, Wu L, Robinson RC, et al. Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily. J Biol Chem. 2010; 285: 40911–40920. pmid:20980257
- View Article
- PubMed/NCBI
- Google Scholar
49. Hiblot J, Bzdrenga J, Champion C, Chabriere E, Elias M. Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia. Sci Rep. 2015; 5: 8372. pmid:25670483
- View Article
- PubMed/NCBI
- Google Scholar

[ref1] 1. Rutherford ST, Bassler BL. Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med. 2012.
View Article
Google Scholar

[2] View Article

[3] Google Scholar

[ref2] 2. Barnard AML, Salmond GPC. Quorum sensing in Erwinia species. Anal Bioanal Chem. 2007; 387: 415–423. pmid:16943991
View Article
PubMed/NCBI
Google Scholar

[5] View Article

[6] PubMed/NCBI

[7] Google Scholar

[ref3] 3. Fuqua WC, Winans SC, Greenberg EP. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J Bacteriol. 1994: 176: 269–275. pmid:8288518
View Article
PubMed/NCBI
Google Scholar

[9] View Article

[10] PubMed/NCBI

[11] Google Scholar

[ref4] 4. Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol. 2001; 55: 165–199. pmid:11544353
View Article
PubMed/NCBI
Google Scholar

[13] View Article

[14] PubMed/NCBI

[15] Google Scholar

[ref5] 5. Von Bodman SB, Bauer WD, Coplin DL. Quorum sensing in plant-pathogenic bacteria. Annu Rev Phytopathol. 2003; 41: 455–482. pmid:12730390
View Article
PubMed/NCBI
Google Scholar

[17] View Article

[18] PubMed/NCBI

[19] Google Scholar

[ref6] 6. Barnard AML, Bowden SD, Burr T, Coulthurst SJ, Monson RE, Salmond GPC. Quorum sensing, virulence and secondary metabolite production in plant soft-rotting bacteria. Philos Trans R Soc Lond B Biol Sci. 2007; 362: 1165–1183. pmid:17360277
View Article
PubMed/NCBI
Google Scholar

[21] View Article

[22] PubMed/NCBI

[23] Google Scholar

[ref7] 7. Burr T, Barnard AML, Corbett MJ, Pemberton CL, Simpson NJL, Salmond GPC. Identification of the central quorum sensing regulator of virulence in the enteric phytopathogen, Erwinia carotovora: the VirR repressor. Mol Microbiol. 2006; 59: 113–125. pmid:16359322
View Article
PubMed/NCBI
Google Scholar

[25] View Article

[26] PubMed/NCBI

[27] Google Scholar

[ref8] 8. Dong Y-H, Zhang L-H. Quorum sensing and quorum-quenching enzymes. J Microbiol. 2005; 43: 101–109. pmid:15765063
View Article
PubMed/NCBI
Google Scholar

[29] View Article

[30] PubMed/NCBI

[31] Google Scholar

[ref9] 9. Dong Y, Zhang X-F, Xu J, Zhang L. Insecticidal Bacillus thuringiensis silences Erwinia carotovora virulence by a new form of microbial antagonism, signal interference. Appl Environ Microbiol. 2004; 70: 954–960. pmid:14766576
View Article
PubMed/NCBI
Google Scholar

[33] View Article

[34] PubMed/NCBI

[35] Google Scholar

[ref10] 10. Pirhonen M, Flego D, Heikinheimo R, Palva ET. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. EMBO J. 1993; 12: 2467–2476. pmid:8508772
View Article
PubMed/NCBI
Google Scholar

[37] View Article

[38] PubMed/NCBI

[39] Google Scholar

[ref11] 11. Bainton NJ, Stead P, Chhabra SR, Bycroft BW, Salmond GP, Stewart GS, et al. N-(3-oxohexanoyl)-L-homoserine lactone regulates carbapenem antibiotic production in Erwinia carotovora. Biochem J. 1992; 288: 997–1004. pmid:1335238
View Article
PubMed/NCBI
Google Scholar

[41] View Article

[42] PubMed/NCBI

[43] Google Scholar

[ref12] 12. Bauer WD, Robinson JB. Disruption of bacterial quorum sensing by other organisms. Curr Opin Biotechnol. 2002; 13: 234–237. pmid:12180098
View Article
PubMed/NCBI
Google Scholar

[45] View Article

[46] PubMed/NCBI

[47] Google Scholar

[ref13] 13. Dong YH, Wang LH, Xu JL, Zhang HB, Zhang XF, Zhang LH. Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase. Nature 2001; 411: 813–817. pmid:11459062
View Article
PubMed/NCBI
Google Scholar

[49] View Article

[50] PubMed/NCBI

[51] Google Scholar

[ref14] 14. Kalia VC. Quorum sensing inhibitors: an overview. Biotechnol Adv. 2013; 31: 224–245. pmid:23142623
View Article
PubMed/NCBI
Google Scholar

[53] View Article

[54] PubMed/NCBI

[55] Google Scholar

[ref15] 15. Czajkowski R, Jafra S. Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules. Acta Biochim Pol. 2009; 56: 1–16. pmid:19287806
View Article
PubMed/NCBI
Google Scholar

[57] View Article

[58] PubMed/NCBI

[59] Google Scholar

[ref16] 16. Lee SJ, Park S, Lee J-J, Yum D, Koo B, Lee J-K. Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis. Appl Environ Microbiol. 2002; 68: 3919–3924. pmid:12147491
View Article
PubMed/NCBI
Google Scholar

[61] View Article

[62] PubMed/NCBI

[63] Google Scholar

[ref17] 17. Dong YH, Xu JL, Li XZ, Zhang LH. AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proc Natl Acad Sci U S A. 2000; 97: 3526–3531. pmid:10716724
View Article
PubMed/NCBI
Google Scholar

[65] View Article

[66] PubMed/NCBI

[67] Google Scholar

[ref18] 18. d’Angelo-Picard C, Faure D, Penot I, Dessaux Y. Diversity of N-acyl homoserine lactone-producing and-degrading bacteria in soil and tobacco rhizosphere. Environ Microbiol. 2005; 7: 1796–1808. pmid:16232294
View Article
PubMed/NCBI
Google Scholar

[69] View Article

[70] PubMed/NCBI

[71] Google Scholar

[ref19] 19. Leadbetter JR, Greenberg EP. Metabolism of acyl-homoserine lactone quorum-sensing signals by Variovorax paradoxus. J Bacteriol. 2000; 182: 6921–6926. pmid:11092851
View Article
PubMed/NCBI
Google Scholar

[73] View Article

[74] PubMed/NCBI

[75] Google Scholar

[ref20] 20. Lin Y-H, Xu J-L, Hu J, Wang L-H, Ong SL, Leadbetter JR, et al. Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes. Mol Microbiol. 2003; 47: 849–860. pmid:12535081
View Article
PubMed/NCBI
Google Scholar

[77] View Article

[78] PubMed/NCBI

[79] Google Scholar

[ref21] 21. Jafra S, Przysowa J, Czajkowski R, Michta a, Garbeva P, van der Wolf JM. Detection and characterization of bacteria from the potato rhizosphere degrading N-acyl-homoserine lactone. Can J Microbiol. 2006; 52: 1006–1015. pmid:17110970
View Article
PubMed/NCBI
Google Scholar

[81] View Article

[82] PubMed/NCBI

[83] Google Scholar

[ref22] 22. Park S-Y, Lee SJ, Oh T-K, Oh J-W, Koo B-T, Yum D-Y, et al. AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria. Microbiology 2003; 149: 1541–1550. pmid:12777494
View Article
PubMed/NCBI
Google Scholar

[85] View Article

[86] PubMed/NCBI

[87] Google Scholar

[ref23] 23. Jones S, Yu B, Bainton NJ, Birdsall M, Bycroft BW, Chhabra SR, et al. The lux autoinducer regulates the production of exoenzyme virulence determinants in Erwinia carotovora and Pseudomonas aeruginosa. EMBO J. 1993; 12: 2477–2482. pmid:8508773
View Article
PubMed/NCBI
Google Scholar

[89] View Article

[90] PubMed/NCBI

[91] Google Scholar

[ref24] 24. McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Camara M, et al. Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 1997; 143: 3703–3711. pmid:9421896
View Article
PubMed/NCBI
Google Scholar

[93] View Article

[94] PubMed/NCBI

[95] Google Scholar

[ref25] 25. Steindler L, Venturi V. Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors. FEMS Microbiol Lett. 2007; 266: 1–9. pmid:17233715
View Article
PubMed/NCBI
Google Scholar

[97] View Article

[98] PubMed/NCBI

[99] Google Scholar

[ref26] 26. Maisuria VB, Patel VA, Nerurkar AS. Biochemical and thermal stabilization parameters of polygalacturonase from Erwinia carotovora subsp. carotovora BR1. J Microbiol Biotechnol. 2010; 20: 1077–1085. pmid:20668400
View Article
PubMed/NCBI
Google Scholar

[101] View Article

[102] PubMed/NCBI

[103] Google Scholar

[ref27] 27. Maisuria VB, Nerurkar AS. Biochemical properties and thermal behavior of pectate lyase produced by Pectobacterium carotovorum subsp. carotovorum BR1 with industrial potentials. Biochem Eng J. 2012; 63: 22–30.
View Article
Google Scholar

[105] View Article

[106] Google Scholar

[ref28] 28. Maisuria VB, Nerurkar AS. Characterization and differentiation of soft rot causing Pectobacterium carotovorum of Indian origin. Eur J Plant Pathol. 2012; 136: 87–102.
View Article
Google Scholar

[108] View Article

[109] Google Scholar

[ref29] 29. Shaw PD, Ping G, Daly SL, Cha C, Cronan JE, Rinehart KL, et al. Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc Natl Acad Sci U S A. 1997; 94: 6036–6041. pmid:9177164
View Article
PubMed/NCBI
Google Scholar

[111] View Article

[112] PubMed/NCBI

[113] Google Scholar

[ref30] 30. Blosser RS, Gray KM. Extraction of violacein from Chromobacterium violaceum provides a new quantitative bioassay for N-acyl homoserine lactone autoinducers. J Microbiol Methods 2000; 40: 47–55. pmid:10739342
View Article
PubMed/NCBI
Google Scholar

[115] View Article

[116] PubMed/NCBI

[117] Google Scholar

[ref31] 31. Winson MK, Swift S, Fish L, Throup JP, Jørgensen F, Chhabra SR, et al. Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing. FEMS Microbiol Lett. 1998; 163: 185–192. pmid:9673021
View Article
PubMed/NCBI
Google Scholar

[119] View Article

[120] PubMed/NCBI

[121] Google Scholar

[ref32] 32. Liao C-H, Shollenberger LM. Enumeration, resuscitation, and infectivity of the sublethally injured erwinia cells induced by mild Acid treatment. Phytopathol. 2004; 94: 76–81.
View Article
Google Scholar

[123] View Article

[124] Google Scholar

[ref33] 33. Sambrook J, Green MR. Molecular cloning- A laboratory manual. 4th ed. Inglis J, Boyle A, Gann A, editors. New York: Cold spring harbour laboratory press. 2012.

[ref34] 34. Huang JJ, Han J, Zhang L, Leadbetter JR. Utilization of acyl-homoserine lactone quorum signals for growth by a soil Pseudomonad and Pseudomonas aeruginosa PAO1. Appl Environ Microbiol. 2003; 69: 5941–5949. pmid:14532048
View Article
PubMed/NCBI
Google Scholar

[127] View Article

[128] PubMed/NCBI

[129] Google Scholar

[ref35] 35. Xu S, Zhao L, Larsson A, Smeds E, Kusche-Gullberg M, Venge P. Purification of a 75 kDa protein from the organelle matrix of human neutrophils and identification as N-acetylglucosamine-6-sulphatase. Biochem J. 2005; 387: 841–847. pmid:15595925
View Article
PubMed/NCBI
Google Scholar

[131] View Article

[132] PubMed/NCBI

[133] Google Scholar

[ref36] 36. Park S, Kang H, Jang H, Lee J, Koo B, Yum D-Y. Identification of extracellular N-acylhomoserine lactone acylase from a Streptomyces sp. and its application to quorum quenching. Appl Environ Microbiol. 2005; 71: 2632–2641. pmid:15870355
View Article
PubMed/NCBI
Google Scholar

[135] View Article

[136] PubMed/NCBI

[137] Google Scholar

[ref37] 37. Morohoshi T, Nakazawa S, Ebata A, Kato N, Ikeda T. Identification and characterization of N-acylhomoserine lactone-acylase from the fish intestinal Shewanella sp. strain MIB015. Biosci Biotechnol Biochem. 2008; 72: 1887–1893. pmid:18603799
View Article
PubMed/NCBI
Google Scholar

[139] View Article

[140] PubMed/NCBI

[141] Google Scholar

[ref38] 38. Romero M, Diggle SP, Heeb S, Cámara M, Otero A. Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase. FEMS Microbiol Lett. 2008; 280: 73–80. pmid:18194337
View Article
PubMed/NCBI
Google Scholar

[143] View Article

[144] PubMed/NCBI

[145] Google Scholar

[ref39] 39. Huang JJ, Petersen A, Whiteley M, Leadbetter JR. Identification of QuiP, the product of gene PA1032, as the second acyl-homoserine lactone acylase of Pseudomonas aeruginosaPAO1. Appl Environ Microbiol. 2006; 72: 1190–1197. pmid:16461666
View Article
PubMed/NCBI
Google Scholar

[147] View Article

[148] PubMed/NCBI

[149] Google Scholar

[ref40] 40. Shepherd RW, Lindow SE. Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology. Appl Environ Microbiol. 2009; 75: 45–53. pmid:18997027
View Article
PubMed/NCBI
Google Scholar

[151] View Article

[152] PubMed/NCBI

[153] Google Scholar

[ref41] 41. Czajkowski R, Krzyżanowska D, Karczewska J, Atkinson S, Przysowa J, Lojkowska E, et al. Inactivation of AHLs by Ochrobactrum sp. A44 depends on the activity of a novel class of AHL acylase. Environ Microbiol Rep. 2011; 3: 59–68. pmid:23761232
View Article
PubMed/NCBI
Google Scholar

[155] View Article

[156] PubMed/NCBI

[157] Google Scholar

[ref42] 42. Holmquist M. Alpha/Beta-hydrolase fold enzymes: structures, functions and mechanisms. Curr Protein Pept Sci. 2000; 1: 209–235. pmid:12369917
View Article
PubMed/NCBI
Google Scholar

[159] View Article

[160] PubMed/NCBI

[161] Google Scholar

[ref43] 43. Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, et al. The alpha/beta hydrolase fold. Protein Eng. 1992; 5: 197–211. pmid:1409539
View Article
PubMed/NCBI
Google Scholar

[163] View Article

[164] PubMed/NCBI

[165] Google Scholar

[ref44] 44. Yates EA, Philipp B, Buckley C, Atkinson S, Chhabra SR, Sockett RE, et al. N-acylhomoserine lactones undergo lactonolysis in a pH-, temperature-, and acyl chain length-dependent manner during growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa. Infect Immun. 2002; 70: 5635–5646. pmid:12228292
View Article
PubMed/NCBI
Google Scholar

[167] View Article

[168] PubMed/NCBI

[169] Google Scholar

[ref45] 45. Wang W-Z, Morohoshi T, Ikenoya M, Someya N, Ikeda T. AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum. Appl Environ Microbiol. 2010; 76: 2524–2530. pmid:20173075
View Article
PubMed/NCBI
Google Scholar

[171] View Article

[172] PubMed/NCBI

[173] Google Scholar

[ref46] 46. Wang L-H, Weng L-X, Dong Y-H, Zhang L-H. Specificity and enzyme kinetics of the quorum-quenching N-Acyl homoserine lactone lactonase (AHL-lactonase). J Biol Chem. 2004; 279: 13645–13651. pmid:14734559
View Article
PubMed/NCBI
Google Scholar

[175] View Article

[176] PubMed/NCBI

[177] Google Scholar

[ref47] 47. Chow JY, Wu L, Wen SY. Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp. paratuberculosis K-10 in the amidohydrolase superfamily. Biochem. 2009; 48: 4344–4353.
View Article
Google Scholar

[179] View Article

[180] Google Scholar

[ref48] 48. Chow JY, Xue B, Lee KH, Tung A, Wu L, Robinson RC, et al. Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily. J Biol Chem. 2010; 285: 40911–40920. pmid:20980257
View Article
PubMed/NCBI
Google Scholar

[182] View Article

[183] PubMed/NCBI

[184] Google Scholar

[ref49] 49. Hiblot J, Bzdrenga J, Champion C, Chabriere E, Elias M. Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia. Sci Rep. 2015; 5: 8372. pmid:25670483
View Article
PubMed/NCBI
Google Scholar

[186] View Article

[187] PubMed/NCBI

[188] Google Scholar

Figures

Abstract

Background

Methodology/Principal Findings

Significance

Introduction

Materials and Methods

Bacterial strains and culture conditions

Isolation, characterization, identification and growth condition of AHL degrading bacteria

Preparation of natural AHL extracts

AHL bioassays

Enzyme assays

Co-cultivation of Pcc BR1 and AHL degrading isolates

Purification and characterization of AHL degrading enzyme

Effect of temperature and pH on enzyme activity and kinetics

HPLC, EI-MS and NMR analyses of AHLs and its degradation product

Results and Discussion

Isolated Delftia sp. VM4 can inactivate different types of AHLs

Purification and characterization of AHL degrading enzyme of Delftia sp. VM4

Biochemical properties of purified AHL acylase from Delftia sp. VM4

Supporting Information

S1 Fig. Phylogenetic analysis of isolate VM4 within the genus Delftia.

S4 Fig. Tryptic digest fingerprint and sequence matches after MALDI-TOF MS/MS analysis of purified AHL acylase from Delftia sp. VM4.

S1 Table. Purification of AHL acylase from Delftia sp. VM4.

Acknowledgments

Author Contributions

References