Journal of Zhengzhou University(Medical Sciences). 2014, (04) Core

Effects of sorafenib on hepatic stellate cell viability and activationChinese Full Text

WANG Lin;LI Bo;GENG Zhimin;XU Zhichao;CHEN Chen;LI Wenzhi;ZHENG Jianbao;Department of Hepatobiliary Surgery,the First Affiliated Hospital,Medical College,Xi’an Jiaotong University;Department of General Surgery,the First Affiliated Hospital,Medical College,Xi’an Jiaotong University;

Abstract: Aim: To investigate effects and mechanisms of sorafenib on hepatic stellate cell（ HSC） viability and activation. Methods: LX2 cells were cultured with 1,2,5,10 μmol /L sorfenib and detected with MTT assay at different time respectively. Expression of α-SMA was measured immunocytochemically in LX2 cells treated with different concentrations of sorafenib. Changes in PDGF-BB and TGF-β1 concentrations were detected in LX2 supernatant using ELISA. Expression of ERK1,ERK2 and AKT signaling pathways was measured using Western blot assay. Results: After treatment with various concentrations of sorafenib for different times,the LX2 inhibition rate increased gradually（ Fgroup= 1 045. 010,Ftime=279. 740,Finteraction= 20. 130,P ＜ 0. 001）. α-SMA expression treated with sorafenib became weaker than that in control group（ H = 35. 630,P ＜ 0. 001）. PDGF-BB and TGF-β1 concentrations decreased with the increasing of sorafenib concentration and time exposured（ P ＜ 0. 05）. ERK1,ERK2 and AKT expression was identical between experimental and control groups,but their phosphorylated expression decreased with increased concentrations of sorafenib,especially in 10 μmol /L sorafenib group. Conclusion: Sorafenib could suppress HSC proliferation and activation.

Keywords：

sorafenib; liver fibrosis; hepatic stellate cell;

DOI：
10.13705/j.issn.1671-6825.2014.04.016
Series：
Subject：
Classification Code：
R575.2

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Effects of sorafenib on hepatic stellate cell viability and activationChinese Full Text

WANG Lin;LI Bo;GENG Zhimin;XU Zhichao;CHEN Chen;LI Wenzhi;ZHENG Jianbao;Department of Hepatobiliary Surgery,the First Affiliated Hospital,Medical College,Xi’an Jiaotong University;Department of General Surgery,the First Affiliated Hospital,Medical College,Xi’an Jiaotong University;

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