Skip to main content
SpringerLink
Log in

LC Analysis of Isepamicin in Plasma Samples Post-Inhalation with Fluorescence Detection and Its Application to a Pharmacokinetic Study

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

A simple and novel LC method has been developed for determination of isepamicin (ISP) in rat plasma, an aminoglycoside antibiotic agent. After protein precipitation and clean-up procedure to remove lipophilic contaminants, ISP is derivatized by pre-column with 9-fluorenylmethyl chloroformate for fluorescence detection. Chromatographic separations are achieved using a C18 column and mobile phase consisting of water and acetonitrile (68/32, v/v). Amikacin was used as an internal standard. The calibration curve was linear over a concentration range of 0.625–15 μg mL−1. The limit of quantification was 0.45 μg mL−1. The intra- and inter-day variabilities of ISP were both less than 5%. Both derivatives were stable for at least a week at ambient condition. This assay procedure should have useful application in therapeutic drug monitoring of ISP. The limit of detection was 0.10 μg mL−1. The specificity, assay linearity, low level assay linearity and assay repeatability were also investigated. The established method provides a reliable bioanalytical method to carry out isepamicin pharmacokinetics in rat plasma.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Hosokawa S, Nakamura K, Fujita Y, Horiuchi R, Yamamoto K (2008) Biol Pharm Bull 31:1866–1869

    Article  CAS  Google Scholar 

  2. Nagabhushan TL, Cooper AB, Tsai H, Daniels PJ, Miller GH (1978) J Antibiot 31:681–687

    CAS  Google Scholar 

  3. Wichert B, Schreier H, Derendorf H (1991) J Pharm Biomed Anal 9:251–254

    Article  CAS  Google Scholar 

  4. Sabath LD, Casey JI, Ruch PA, Stumpf LL, Finland M (1971) J Lab Clin Med 78:457–463

    CAS  Google Scholar 

  5. Flurer CL, Wolnik KA (1994) J Chromatogr A 663:259–263

    Article  CAS  Google Scholar 

  6. Calcara M, Enea V, Pricoco A, Miano F (2005) J Pharm Biomed Anal 38:344–348

    Article  CAS  Google Scholar 

  7. Bhogte CP, Patravale VB, Devarajan PV (1997) J Chromatogr B 694:443–447

    Article  CAS  Google Scholar 

  8. Mayhew JW, Gorbach SLG (1978) J Chromatogr 151:133–146

    Article  CAS  Google Scholar 

  9. Vogel R, DeFillipo K, Reif V (2001) J Pharm Biomed Anal 24:405–412

    Article  CAS  Google Scholar 

  10. Kaine LA, Wolnik KA (1994) J Chromatogr A 674:255–261

    Article  CAS  Google Scholar 

  11. Adams E, Liu L, Dierick E, Guyomard S, Nabet P, Rico S, Louis P, Roets E, Hoogmartens J (1998) J Pharm Biomed Anal 17:757–766

    Article  CAS  Google Scholar 

  12. Pastore P, Gallina A, Magno F (2000) Analyst 125:1955–1958

    Article  CAS  Google Scholar 

  13. McLaughlin LG, Henion JD, Kijak PJ (1994) J Biol Mass Spectrom 23:417–429

    Article  CAS  Google Scholar 

  14. Hu P, Chess EK, Brynjelsen S, Jakubowski G, Melchert J, Hammond Arb (2000) J Am Soc Mass Spectrom 11:200–209

    Article  CAS  Google Scholar 

  15. Loffler D, Ternes TA (2003) J Chromatogr A 1000:583–588

    Article  CAS  Google Scholar 

  16. Pathak SM, Kumar AR, Musmade P, Udupa N (2008) Talanta 76:338–346

    Article  CAS  Google Scholar 

  17. Mashat M, Chrystyn H, Clark BJ, Assi KH (2008) J Chromatogr B 69:59–66

    Article  Google Scholar 

  18. Kim BH, Kim YK, Ok LH (2001) J Chromatogr B 752:173–177

    Article  CAS  Google Scholar 

  19. Hornish RE, Wiest JR (1998) J Chromatogr A 812:123–133

    Article  CAS  Google Scholar 

  20. Fishermen N, Soback S (2000) Clin Chem 46:837–842

    Google Scholar 

  21. Zhou M, Wei G, Liu Y, Sun Y, Xiao S, Lu L, Liu C, Zhong D (2003) J Chromatogr B 798:43–48

    Article  CAS  Google Scholar 

  22. Liu Z, Sha Y, Huang T, Yang B, Duan G-L (2005) J Chromatogr B 828:2–8

    Article  CAS  Google Scholar 

  23. Peng GW, Jackson GG, Chiou WL (1977) Antimicrob Agents Chemother 12:707–709

    CAS  Google Scholar 

  24. Suhren G, Knappstein K (1998) Analyst 123:2797–2801

    Article  CAS  Google Scholar 

  25. Wang J, Hong LY, Ni KY (2006) Chin J Anal Chem 34:19–22

    Google Scholar 

  26. Yuan JB, Nie LH, Zeng DY, Luo XB, Tang F, Ding L, Liu Q, Guo ML, Yao SZ (2007) Talanta 73:644–650

    Article  CAS  Google Scholar 

  27. Almeida AM, Castel-Branco MM, Falcao AC (2002) J Chromatogr B 774:215–222

    Article  CAS  Google Scholar 

  28. Yuan C, Jia N, Wang JX, Liu SF (1999) Chin J Pharm Anal 19:108–111

    CAS  Google Scholar 

  29. Xian D (2005) J Chongqing Med 5:987–990

    Google Scholar 

  30. Yang WC, Yu AM, Chen HY (2001) J Chromatogr A 905:309–318

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This project is supported by the Chongqing Municipal Key Laboratory on Luminescence and Real-Time Analysis, Southwest University (no. CSTC2006CA 8006). All authors here express their deep thanks.

Author information

Authors and Affiliations

  1. Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, Chemistry and Chemical Engineering, Southwest University, 400715, Chongqing, China

    Xiaojuan Chang & Jingdong Peng

Authors
  1. Xiaojuan Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jingdong Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jingdong Peng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, X., Peng, J. LC Analysis of Isepamicin in Plasma Samples Post-Inhalation with Fluorescence Detection and Its Application to a Pharmacokinetic Study. Chroma 70, 1429–1433 (2009). https://doi.org/10.1365/s10337-009-1331-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-009-1331-5

Keywords

Search

Navigation