Abstract
A simple, rapid and sensitive liquid chromatographic method using a refractive index detector has been developed and validated for identification and quantification of β-cyclodextrin in milk, cream and butter. The chromatographic system consists of a YMC ODS-AQ column packed with C18 reversed phase silica packing material as stationary phase using a mixture of methanol and water 7:93% (v/v) as the mobile phase. Linearity was established for the β-cyclodextrin concentration in the range 0.01–4 mg mL−1, with a coefficient (r) of 0.9998. Repeatability of the method was assessed; the coefficient of variation for β-cyclodextrin range were 1.24, 3.01, and 5.36% for milk, cream and butter. Recoveries ranged between 99.91 and 94.53%. This method was highly reproducible and reliable for quantification of treated milk with β-cyclodextrin for removal cholesterol from the milk fat.
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Acknowledgments
Dr. L. Alonso thanks the Ministry of Education and Science of Spain for the support during his time in the Food and Agricultural Product Research and Technology Center at Oklahoma State University, USA and L. Alonso would especially like to thank Dr. Gilliland for providing the facilities, helpful suggestions and joining in his research group.
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An erratum to this article can be found at http://dx.doi.org/10.1365/s10337-009-1095-y
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López, L.A., Alonso, P.C. & Gilliland, S.E. Analytical Method for Quantification β-Cyclodextrin in Milk, Cream and Butter by LC. Chroma 69, 1089–1092 (2009). https://doi.org/10.1365/s10337-009-1022-2
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DOI: https://doi.org/10.1365/s10337-009-1022-2