Abstract
Carvedilol was quantified in human plasma after a single 25 mg oral dose of the drug was given to 26 healthy volunteers. An applied Biosystems Sciex API 3200 triple quadrupole mass spectrometer in multiple reaction monitoring mode, using electrospray ionization in the positive ion mode was used. Simple solid phase extraction was followed by C18 reverse phase chromatography and tandem mass spectrometric detection using propranalol as the internal standard. The mean recovery for carvedilol was 91.2% with a lower limit of quantification of 0.5 ng mL−1. This validated assay method makes use of the increased sensitivity and selectivity of tandem mass spectrometry detection to allow for a more rapid and selective method for the determination of carvedilol in human plasma.
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References
Therapeutic Drugs (1999) 2nd edn. Colin Dollery, Churchill Livingstone Co., pp C75–C80
Hokama N, Hobara N, Kameya H, Ohshiro S, Sakanashi M (1999) J Chromatogr B 732(1):233–238. doi:10.1016/S0378-4347(99)00248-0
Leung GNW, Leung DKK, Terence SM, Colton W, Wong HF (2007) J Chromatogr A 1156(1–2):271–279. doi:10.1016/j.chroma.2006.10.006
Maiko M, Masato W, Shigeru T, Shigeo K, Akikazu N (2003) J Chromatogr B Analyt Technol Biomed Life Sci 798(2):187–191. doi:10.1016/j.jchromb.2003.09.039
Rathod R, Prasad LPC, Rani S, Nivsarkar M, Padh H (2007) J Chromatogr B 857(2):219–223. doi:10.1016/j.jchromb.2007.07.021
Zarghi A, Foroutan SM, Shafaati A, Khoddam A (2007) J Pharm Bio An 44(1):250–253. doi:10.1016/j.jpba.2007.01.026
Eisenberg EJ, Patterson WR, Kahn GC (1989) J Chromatogr B Analyt Technol Biomed Life Sci 493:105–115. doi:10.1016/S0378-4347(00)82713-9
Medvedovici A, Albu F, Georgita C, Sora DI, Galaon T, Udrescu S, David V (2007) J Chromatogr B 850(1–2):327–335. doi:10.1016/j.jchromb.2006.12.004
Saito M, Kawana J, Ohno T, Kaneko M, Mihara K, Hanada K, Sugita R, Okada N, Oosato S, Nagayama M, Sumiyoshi T, Ogata H (2006) J Chromatogr B 843(1):73–77. doi:10.1016/j.jchromb.2006.05.018
Borges NC, Mendes GD, Silva DO, Rezende VM, Barrientos-Astigarraga RE, De Nucci G (2005) J Chromatogr B 822(1–2):253–262. doi:10.1016/j.jchromb.2005.06.012
Li S, Liu G, Jia J, Liu Y, Pan C, Yu C, Cai Y, Ren J (2007) J Chromatogr B 847(2):174–181. doi:10.1016/j.jchromb.2006.10.013
Yang E, Wang S, Kratz J, Cyronak MJ (2004) J Pharm Bio An 36(3):609–615. doi:10.1016/j.jpba.2004.07.008
Jeong DW, Kim YH, Ji HY, Youn YS, Lee KC, Lee HS (2007) J Pharm Bio An 44(2):547–552. doi:10.1016/j.jpba.2006.12.017
Ptácek P, Macek J, Klíma J (2003) J Chromatogr B Analyt Technol Biomed Life Sci 789(2):405–410. doi:10.1016/S1570-0232(03)00078-3
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Gomes, N.A., Vaidya, V.V., Pudage, A.M. et al. Application of a Sensitive, Rapid and Validated LC–MS–MS Method for the Determination of Carvedilol in Human Plasma. Chroma 69, 19–25 (2009). https://doi.org/10.1365/s10337-008-0848-3
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DOI: https://doi.org/10.1365/s10337-008-0848-3