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Quantitative Determination of Octylonium in Human Plasma by LC–MS

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Abstract

The purpose of this investigation was to develop a method for measuring the concentration of octylonium in human plasma. Hydrochloric acid was added to the plasma samples before pretreatment to improve the stability of the octylonium. After liquid–liquid extraction with ethylacetate and isopropanol (10:1), the analytes were separated by chromatography on a reversed-phase C18 column and detected by LC–MS–MS with electrospray ionization–ionization. The coefficient of variation for the precision of the assay was less than 10.1%, and the accuracy ranged from 98.0 to 106.5%. The limit of quantification or sensitivity was 0.2 ng mL−1. This method was validated by measuring octylonium in the plasma of healthy human subjects after administration of a single 120-mg oral dose of octylonium bromide. Thus, a highly sensitive and accurate analytical method was developed to determine the concentration of octylonium in human plasma.

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Acknowledgements

This work was supported by a grant of the Korea Food and Drug Administration (KFDA2006–7052).

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Authors and Affiliations

  1. College of Pharmacy, Catholic University of Daegu, Kyoungbuk, 712–702, South Korea

    Kibum Kim, Haehum Jung & Wonku Kang

  2. College of Pharmacy, Chungnam National University, Daejeon, 305–764, South Korea

    Hyi-Yoel Yun & Kwang-il Kwon

  3. College of Medicine, Kyoungbuk National University, Daegu, 702–701, South Korea

    Seung-Ok Moon & Young-Ran Yoon

  4. Korea Food and Drug Administration, Seoul, 122–704, South Korea

    Hyesoo Kim & Soojung Shon

Authors
  1. Kibum Kim
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  2. Haehum Jung
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  3. Hyi-Yoel Yun
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  4. Seung-Ok Moon
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  5. Young-Ran Yoon
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  6. Kwang-il Kwon
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  7. Hyesoo Kim
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  8. Soojung Shon
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  9. Wonku Kang
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Corresponding author

Correspondence to Wonku Kang.

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Kim, K., Jung, H., Yun, HY. et al. Quantitative Determination of Octylonium in Human Plasma by LC–MS. Chroma 66, 257–260 (2007). https://doi.org/10.1365/s10337-007-0274-y

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  • DOI: https://doi.org/10.1365/s10337-007-0274-y

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