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HPTLC Determination of Atorvastatin in Plasma

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Abstract

High performance liquid chromatography has been the most popular choice for the determination of atorvastatin. In this study, two-step isocratic chromatography on silica gel 60F254 HPTLC layer and densitometric quantitation at λ = 280 nm was developed for the separation of atorvastatin from plasma constituencies and sodium diclofenac as peak-tracer. The established HPTLC method was validated in terms of LOD/LOQ, linearity, recovery and repeatability. The calibration function of the analyte was linear in the range 101–353.5 ng zone−1 and the correlation coefficient was 0.9969. The limits of detection and quantitation were 30.3 and 101 ng zone−1. The recovery and relative standard deviation obtained from between-days analysis were 97.5–103.0 and 1.7–3.4%.

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Authors and Affiliations

  1. Department of Novel Drug Delivery Systems, Iran Polymer and Petrochemical Institute, P.O. Box 14185/458, Tehran, Iran

    A. Jamshidi

  2. Polymer Technology Incubators, Iran Polymer and Petrochemical Institute, P.O. Box 14185/458, Tehran, Iran

    A. R. Nateghi

Authors
  1. A. Jamshidi
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  2. A. R. Nateghi
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Correspondence to A. Jamshidi.

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Jamshidi, A., Nateghi, A.R. HPTLC Determination of Atorvastatin in Plasma. Chroma 65, 763–766 (2007). https://doi.org/10.1365/s10337-007-0228-4

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  • DOI: https://doi.org/10.1365/s10337-007-0228-4

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