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Effects of Mobile Phase Ratios on the Separation of Plant Carotenoids by HPLC with a C30 Column

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Abstract

Plant products are dietary sources of lutein and zeaxanthin. Lutein and zeaxanthin have been implicated in the protection of age related macular degeneration (AMD) and in cardiovascular diseases. However, xanthophylls and unidentified components (λmax = 423 and 468 nm) in plant products are often not separated well, and affect an accurate quantitative determination of lutein and zeaxanthin. A high performance liquid chromatography (HPLC) system equipped with a Bischoff C30 column and a mobile phase of methanol, methyl-tert-butyl ether (MTBE) and water was used to separate lutein, zeaxanthin and other unidentified components in plant products. Mobile phase A containing methanol, MTBE and water with a ratio of 60:33:7 by volume (1.5% ammonium acetate, NH4Ac), combined with mobile phase B with a ratio of 8:90:2 by volume (1.0% NH4Ac) is optimal for the separation. This method was successfully applied to the quantitative determination of lutein and zeaxanthin in extracts of plant products, such as chlorella, spirulina, celery and mallow.

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Acknowledgements

The authors are grateful to the Nestle Nutrition Scholarship and Nestle Research Foundation for their grant support to this research project.

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Authors and Affiliations

  1. Department of Maternal and Child Nutrition, Nutrition and Food Safety Institute, Chinese Center for Disease Control and Prevention, Beijing, 100050, China

    Lei Li & Shi-an Yin

  2. Carotenoids and Health Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging (HNRCA), Tufts University, Boston, 02111, USA

    Jian Qin & Guangwen Tang

Authors
  1. Lei Li
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  2. Jian Qin
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  3. Shi-an Yin
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  4. Guangwen Tang
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Correspondence to Guangwen Tang.

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Li, L., Qin, J., Yin, Sa. et al. Effects of Mobile Phase Ratios on the Separation of Plant Carotenoids by HPLC with a C30 Column. Chroma 65, 91–94 (2007). https://doi.org/10.1365/s10337-006-0100-y

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  • DOI: https://doi.org/10.1365/s10337-006-0100-y

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