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Chromatographic Fingerprint Analysis of Macrothelypteris torresiana and Simultaneous Determination of Several Main Constituents by LC

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Abstract

A simple and reliable identification and quality control system has been developed for monitoring a herbal plant (Macrothelypteris torresiana) based on quantitative fingerprinting analysis using liquid chromatographic method with ultra-violet detector. The herbal extract was obtained by ultrasonic-assisted extraction. The separation was performed on a Burospher-100 C18 column by gradient elution with acetonitrile and aqueous phase (containing 0.5% H3PO4, pH 3.0) at a flow rate of 1.0 mL min−1. Under the optimal chromatographic condition, the relative standard deviations of the retention time and the peak area were less than 0.39 and 5.51%, respectively. The intra- and inter-day precisions were ranged from 0.76 to 3.23%. Good linear behaviors over the investigated concentration ranges were obtained with the values of R 2 higher than 0.999 for all analytes. The recoveries for spiked samples were in the range of 92.2–106.0%. Eleven peaks in the chromatograms of M. torresiana were identified for chromatographic fingerprint analysis. The proposed method was successfully applied to determine the contents of main constituents (protoapigenone, protoapigenin 4′-O-β-D-glucoside, apigenin 4′-O-β-D-glucoside, and apigenin) in different batches of M. torresiana. The proposed analytical procedure was proved to be a reliable and rapid method for the identification and quality control of the herbal plant M. torresiana.

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Authors and Affiliations

  1. Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei Province, College of Pharmacy, Tongji Medical Center, Huazhong University of Science and Technology, Wuhan, People’s Republic of China

    Chaomei Xiong, Jinlan Ruan, Ying Tang, Yaling Cai, Wei Fang, Yong Zhu & Daonian Zhou

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  1. Chaomei Xiong
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  2. Jinlan Ruan
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  3. Ying Tang
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  4. Yaling Cai
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  5. Wei Fang
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  6. Yong Zhu
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  7. Daonian Zhou
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Correspondence to Jinlan Ruan.

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Xiong, C., Ruan, J., Tang, Y. et al. Chromatographic Fingerprint Analysis of Macrothelypteris torresiana and Simultaneous Determination of Several Main Constituents by LC. Chroma 70, 117–124 (2009). https://doi.org/10.1365/s10337-009-1115-y

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  • DOI: https://doi.org/10.1365/s10337-009-1115-y

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