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Stability-Indicating LC–PDA Method for Determination of Picrosides in Hepatoprotective Indian Herbal Preparations of Picrorhiza kurroa

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Abstract

A liquid chromatography method was developed and validated for the determination of picroside-I and picroside-II in herbal preparations containing Picrorhiza kurroa as one of the ingradients. Resolution of picrosides was achieved on a reversed phase (C-18) endcapped bidentate column by using a mobile phase of acetonitrile: water (25:75 v/v). The detection of picrosides was carried out at 270 nm. The method was validated for precision, accuracy and robustness according to the International Conference on Harmonization (ICH) guidelines and is applicable for the quality control of preparations containing P. kurroa. Analysis of samples in forced degradation proved it to be applicable for stability evaluation. The linear regression analysis data showed good linear relationship (r 2 = 0.9999 ± 0.0010 for picroside-I and 0.9997 ± 0.0012 for picroside-II) in the concentration range of 0.4–4.0 μg. The limit of detection and quantification for picroside-I and picroside-II were recorded to be 28.1 and 73.1 ng and 85.2 and 221.5 ng, respectively. Satisfactory recovery results were observed from the herbal preparations (97.5–100.5%). Intra- and inter- day precision of the method was acceptable, with relative standard deviation (%RSD) values in the range of 0.04–1.16% and 0.03–0.27%, respectively.

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References

  1. Nadkarni AK (1954) Indian Materica, vol vol. 1. Popular Book Dept, Bombay, p 25–27, 619–622, 634–651, 953–955, 1220–1221, 1252–1253

    Google Scholar 

  2. Ansari RA, Oswal BS, Chandra R, Dhawan BN, Garg NK, Kapoor NK, Kulshrestha DK, Mendhi H, Mehrotra BN, Patnaik GK, Sharma SK (1988) J Med Res 47:401–404

    Google Scholar 

  3. Chaturvedi GN, Singh RH (1996) J Res Indian Med 1:1–14

    Google Scholar 

  4. Langer JG, Gupta OP, Atal CK (1981) Indian J Pharmacol 13:98–99

    Google Scholar 

  5. Kirtikar KR, Basu BD (1935) Indian medicinal plants, vol III. Lalit Mohan Basu, Allahabad, pp 1825–1826

    Google Scholar 

  6. Dey AC, Bhishen S, Mahendra PS (1980) Indian medicinal plants used in ayurvedic preparations. Dehradun, India. J Res Indian Med p 81

  7. Kumar SHS, Anandan R, Devki T, Kumar MS (2001) Fitoterapia 72:402–405. doi:10.1016/S0367-326X(01)00264-7

    Article  Google Scholar 

  8. Atal CK, Sharma ML, Kaul A, Khjuria A (1986) J Ethnopharmacol 18:133–141. doi:10.1016/0378-8741(86)90025-5

    Article  CAS  Google Scholar 

  9. Puri A, Saxena RP, Sumti Guru PY, Kulshrestha DK, Dhawan BN (1992) Planta Medica 58:519–523. doi:10.1055/s-2006-961542

    Article  Google Scholar 

  10. Sharma ML, Rao CS, Duda PL (1994) J Ethnopharmacol 41:185–192. doi:10.1016/0378-8741(94)90031-0

    Article  CAS  Google Scholar 

  11. Kitagawa I, Hino K, Nishimura T, Iwata E, Yosioka I (1971) Chem Pharm Bull (Tokyo) 19:2534–2544

    CAS  Google Scholar 

  12. Weinges K, Kloss P, Henkels WD (1972) Anal Chem 759:173–182

    CAS  Google Scholar 

  13. Halliwel B, Gutteridge MC (1999) Free Radicals in biology and medicine. In study of generalized light emission, 3rd edn. University Press, Oxford, p 387

    Google Scholar 

  14. Singh GB, Bani S, Singh S, Khajuria A, Sharma ML, Gupta BD, Banerjee SK (1993) Phytother Res 7:402–407. doi:10.1002/ptr.2650070604

    Article  CAS  Google Scholar 

  15. Joy KL, Kuttan R (1999) J Ethnopharmacol 167:143–148. doi:10.1016/S0378-8741(98)00243-8

    Article  Google Scholar 

  16. Vaidya AB, Antakar DS, Doshi JC, Bhatt AD, Ramesh V, Vora PV, Perissond D, Baxi AJ, Kale PM (1996) J Postgrad Med 42:105–108

    CAS  Google Scholar 

  17. Sturm S, Stuppner H (2000) Phytochem Anal 11:121–127 10.1002/(SICI)1099-1565(200003/04)11:2<121::AID-PCA493>3.0.CO;2-9

    Article  CAS  Google Scholar 

  18. Stuppner H, Wagner H (1992) Sci Pharm 60:73–85

    CAS  Google Scholar 

  19. Kumar V, Mehrotra N, Lal J, Gupta RC (2004) J Chromatogr A 1045:145–152. doi:10.1016/j.chroma.2004.06.021

    Article  CAS  Google Scholar 

  20. Singh N, Gupta AP, Singh B, Kaul VK (2005) J Liq Chromatgr Relat Tecchnol 28:1679–1691. doi:10.1081/JLC-200060439

    Article  CAS  Google Scholar 

  21. Bansal M, Sharma PK, Jain GK, Mehrotra N (2007) Intern. J Chem Sci 5(2):852–866

    CAS  Google Scholar 

  22. Shan C, Haung J, Junqi H (2007) Pharmazie 62(8):577–579

    Google Scholar 

  23. Sturm S, Stuppner H (2001) Chromatographia 53:612–618. doi:10.1007/BF02493007

    Article  CAS  Google Scholar 

  24. Chauhan SK, Singh BP, Agrawal SR (1999) Indian Drugs 36:604–605

    CAS  Google Scholar 

  25. Dwivedi AK, Chaudhry M, Seth RK, Sarin J (1968) Indian J Pharm Sci 51:274–275

    Google Scholar 

  26. Bhandari P, Kumar N, Singh B, Kaul VK (2008) J Chrom A 1194:254–261. doi:10.1016/j.chroma.2008.04.062

    Article  Google Scholar 

  27. Kumar V, Mehrotra N, Gupta RC (2005) J Chrom B 820:221–227. doi:10.1016/j.jchromb.2005.03.032

    Article  Google Scholar 

  28. Singh S, Singh B, Bahuguna R, Wadhwa L, Saxena R (2006) J Pharm Biomed Anal 41:1037–1040. doi:10.1016/j.jpba.2006.01.030

    Article  CAS  Google Scholar 

  29. Mohammadi A, Haririan I, Rezanour N, Ghiasi L, Walker RB (2006) J Chromatogr A 1116:153–157. doi:10.1016/j.chroma.2006.03.038

    Article  CAS  Google Scholar 

  30. Ivana I, Ljiljana Z, Mira Z (2006) J Chromatogr A 1119:209–215. doi:10.1016/j.chroma.2005.12.079

    Article  CAS  Google Scholar 

  31. Annon., ICH, Q1A(R2) Stability testing of new drug substances and products. In: International Conference on Harmonization, IFPMA, Geneva, 2003

  32. Grosa G, Grosso ED, Russo R, Allegrone G (2006) J Pharm Biomed Anal 41:798–803. doi:10.1016/j.jpba.2006.01.026

    Article  CAS  Google Scholar 

  33. Mishal A, Sober D (2005) J Pharm Biomed Anal 39:819–823. doi:10.1016/j.jpba.2005.05.029

    Article  CAS  Google Scholar 

  34. Singh B, Rastogi RP (1972) Indian J Chem 10:29–31

    Google Scholar 

  35. Jia Q, Hong MF, Minter D (1999) J Nat Prod 62:901–903. doi:10.1021/np980493+

    Article  CAS  Google Scholar 

  36. Stuppner H, Wagner H (1989) Planta Medica 55:467–469. doi:10.1055/s-2006-962066

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Authors are grateful to the CSIR-EMR for awarding the senior research fellowship to Ms. P.B. and technical assistance from Mr. Ramesh Kumar is gratefully acknowledged.

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Authors and Affiliations

  1. Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India

    Pamita Bhandari, Neeraj Kumar, Bikram Singh, Ajai P. Gupta, Vijay K. Kaul & Paramvir S. Ahuja

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  1. Pamita Bhandari
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  2. Neeraj Kumar
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  3. Bikram Singh
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  4. Ajai P. Gupta
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Correspondence to Bikram Singh.

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Bhandari, P., Kumar, N., Singh, B. et al. Stability-Indicating LC–PDA Method for Determination of Picrosides in Hepatoprotective Indian Herbal Preparations of Picrorhiza kurroa . Chroma 69, 221–227 (2009). https://doi.org/10.1365/s10337-008-0889-7

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