Abstract
A gas capillary chromatography of silylated carbohydrates on SPB-17 phase (50% diphenyl/50% dimethylsiloxane) method for the determination of lactulose has been developed. The method has been evaluated for precision and accuracy using phenyl-β-D-glucoside as internal standard with satisfactory results and, then, applied to 27 commercial milk samples (pasteurized, UHT, sterilized, powder, condensed and chocolate-based milks). Results showed that it was suitable for the determination of lactulose in milks subjected to heat treatments of different intensity, giving good chromatographic resolution, as well as precise and reproducible results. Thus, lactulose levels found in pasteurised milk (n = 6) ranged from 13.0 – 32.1 mg L−1, UHT milk (n = 10) ranged from 95.6–437.1 mg L−1, sterilized milk (n = 2) above 622.7 mg L−1 and reconstituted powder milk (n = 3) ranged from 24.2 to 48.8 mg L−1 were similar to those previously reported. In addition, this method provided a good separation between sucrose and lactulose/lactose peaks which allowed the suitable quantification of lactulose in samples such as condensed and chocolate-based milks which contain a high concentration of sucrose.
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References
Adachi S, Patton S (1961) J Dairy Sci 44:1375–1393
European Commission (EC) (1992) Dairy Chemists’ Group-Doc VI/5726/92, Project de decision de la commission fixant les limites et les methods permettant de distinguer les differents types de laits de consummations traits thermiquement
International Dairy Federation (IDF) B-Doc 235, Influence of Technology on the quality of heated milk and fluid milk products, 1993
Geier H, Klostermeyer (1983) Milchwissenschaft 38:475–477
Andrews GR (1984) J Soc Dairy Technol 37: 92–95
Corzo N, Olano A, Martínez-Castro I (1986) Rev Agroquím Tecnol Aliment 26:565–570
Villamiel M, Arias M, Corzo N, Olano A (1999) Z Lebensm Unters Forsch A 208:169–171
Marconi E, Messia MC, Amine A, Moscone D, Vernazza F, Stocchi F, Palleschi G (2004) Food Chem 84:447–450
Olano A, Calvo MM, Reglero G (1986) Chromatographia 21:538–540
International Dairy Federation (IDF) Standard 147. Heat-treated milk. Determination of lactulose content, High Performance Liquid Chromatography (Reference Method), 1991
International Dairy Federation (IDF) Standard 175. Determination of lactulose content, Enzymatic Method, 1995
Amine A, Moscone D, Bernardo RA, Marconi E, Palleschi G (2000) Anal Chim Acta 406:217–224
Ersser RS, Mitchell JD (1984) J Chromatogr 307:393–398
Reimerdes EH, Rothkitt KD (1985) Z Lebensm Unters Forsch 181:408–411
Martínez-Castro I, Calvo MM, Olano A (1987) Chromatographia 23:132–136
Haverkamp J, Kamerling JP, Vliegenthart JFG (1971) J Chromatogr 59:281–287
Martínez-Castro I, Olano A (1978) Rev Esp Lechería 110:213–217
Martínez-Castro I, Olano A (1980) Milchwissenschaft 35:5–8
Olano A, Martinez-Castro I (1981) Milchwissenschaft 36:533–536
de Rafael D, Calvo MM, Olano A (1996) Milchwissenschaft 51:552–553
García-Raso A, Páez MI, Martínez-Castro I, Sanz J, Calvo MM (1992) J Chromatogr 607:221–225
Nikolov ZL, Reilly PJ (1983) J Chromatogr 254:157–162
Olano A, Calvo MM, Corzo N (1989) Food Chem 31:259–265
Andrews G (1989) Bulletin of the International Dairy Federation 238:45–52
Corzo N, Delgado T, Troyano E, Olano A, (1994) J Food Prot 57:737–739
Pellegrino L, De Noni I, Resmini P (1995) Int Dairy J 5:647–659
Akalin AS, Gönc S (1997) Milchwissenschaft 52:377–380
Valero E, Villamiel M, Miralles B, Sanz J, Martínez-Castro I (2001) Food Chem 72:51–58
Rada Mendoza M, Olano A, Villamiel M, (2005) J Agric Food Chem 53:2995–2999
Olano A, Martínez-Castro I (1989) Bulletin of the International Dairy Federation 238:35–44
Hurrell RF, Finot PA, Ford JE (1983) Brit J Nutr 49:343–354
Resmini P, Pellegrino L, Masotti F, Tirelli A, Prati F (1992) Sci Tec Latt-Cas 43:169–186
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Montilla, A., Moreno, F.J. & Olano, A. A Reliable Gas Capillary Chromatographic Determination of Lactulose in Dairy Samples. Chroma 62, 311–314 (2005). https://doi.org/10.1365/s10337-005-0628-2
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DOI: https://doi.org/10.1365/s10337-005-0628-2