Abstract
For the first time a sensitive, specific and rapid LC–MS–MS assay is presented for the simultaneous determination of levodopa (L-DP), 3-O-methyldopa (3-OMD) and benserazide (BSZ) in human serum. The three compounds were extracted from human serum by protein precipitation followed by dilution of the supernatant with aqueous formic acid. In serum, linearity was observed between 50 and 1,000 ng mL−1 of L-DP, 3-OMD and BSZ, respectively. Intra-day and inter-day RSD values were below 10.56 and 6.22% at concentrations of 120, 360 and 720 ng mL−1. The presented method showed excellent specificity and sensitivity compared with other methods reported. It was applied to a pharmacokinetic study and demonstrated its applicability to pre-clinical and clinical pharmacological research.
Similar content being viewed by others
References
Muzzi C, Bertocci E, Terzuoli L, Porcelli B, Ciari I, Pagani R, Guerranti R (2008) Biomed Pharmacother 62:253–258
Marques KL, Santos JL, Lopes JA, Lima JL (2008) Anal Sci 24:985–991
Nunes T, Machado R, Rocha JF, Fernandes-Lopes C, Costa R, Torrão L, Loureiro AI, Falcão A, Vaz-da-Silva M, Wright L, Almeida L, Soares-da-Silva P (2009) Clin Ther 31:2258–2271
Blandini F, Martignoni E, Pacchetti C, Desideri S, Rivellini D, Nappi G (1997) J Chromatogr B Anal Technol Biomed Life Sci 700:278–282
Michotte Y, Moors M, Deleu D, Herregodts P, Ebinger G (1987) J Pharm Biomed Anal 5:659–664
Igarashi K, Hotta K, Kasuya F, Abe K, Sakoda S (2003) J Chromatogr B Anal Technol Biomed Life Sci 792:55–61
Davidson AG (1985) J Pharm Biomed Anal 3:235–240
Hassib ST (1990) Anal Lett 23:2195–2214
Uslua B, Özkana SA (2002) Anal Lett 35:303–314
Karpińska J, Smyk J, Wołyniec E (2005) Spectrochim Acta A 62:213–220
Pistonesi M, Centurión ME, Fernández Band BS, Damiani PC, Olivieri AC (2004) J Pharm Biomed Anal 36:541–547
Coello J, Maspoch S, Villegas N (2000) Talanta 53:627–637
Wu G, Furlanut M (1997) Pharmacol Res 35:553–556
Andreoli R, Manini P, Bergamaschi E, Mutti A, Franchini I, Niessen WM (1999) J Chromatogr A 847:9–17
Lee MS, Kerns EH (1999) Mass Spectrom Rev 18:187–279
Crevoisier C, Monrealc A, Metzgerc NilsenT (2003) Eur Neurol 49:39–44
Zhang D, Wang Y, Han J, Yu W, Deng L, Fawcett JP, Liu Z, Gu J (2009) J Chromatogr B Anal Technol Biomed Life Sci 877:581–585
Juan-García A, Font G, Picó Y (2007) Electrophoresis 28:4180–4191
Yüzüak N, Özden T, Erenl S, Toptan S (2007) Chromatographia 66:115–118
Damen CW, Rosing H, Tibben MM, van Maanen MJ, Lagas JS, Schinkel AH, Schellens JH, Beijnen JH (2008) J Chromatogr B Anal Technol Biomed Life Sci 868:102–109
Matuszewski BK, Constanzer ML, Chavez-Eng CM (2003) Anal Chem 75:3019–3030
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Pan, L., Guo, Y., Li, Z. et al. Simultaneous Determination of Levodopa, Benserazide and 3-O-Methyldopa in Human Serum by LC–MS–MS. Chroma 72, 627–633 (2010). https://doi.org/10.1365/s10337-010-1683-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-010-1683-x