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Rapid Determination of Carbamazepine in Human Urine, Plasma Samples and Water Using DLLME followed by RP–LC

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Abstract

A simple, rapid and efficient method, dispersive liquid–liquid microextraction has been developed for the extraction and preconcentration of carbamazepine in biological samples. Factors influencing microextraction efficiencies, such as the kind and volume of extraction and dispersive solvents, pH and salt effect have been optimized. Under the optimum conditions, the enrichment factor and extraction recovery were 90.7 and 54.4% respectively at the level of 100 μg L−1 of carbamazepine in 5 mL sample volume. The calibration curve was linear in the range of 0.0005–0.2 μg mL−1 and the detection limit was 0.0002 μg mL−1. The relative standard deviation for five replicate measurements of carbamazepine was 7.1%. The relative recoveries of carbamazepine, in plasma, urine and tap water samples at three different spiking levels ranged from 78.6 to 98.5%. The quantitative determination has been made by LC in biological fluids and water samples.

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Authors and Affiliations

  1. Science and Research Branch, Department of Chemistry, Faculty of Science, Islamic Azad University, P.O. Box 1415-775, Tehran, Iran

    Hossein Ali Mashayekhi, Parviz Abroomand-Azar, Mohammad Saber-Tehrani & Syed Waqif Husain

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  1. Hossein Ali Mashayekhi
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  2. Parviz Abroomand-Azar
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  3. Mohammad Saber-Tehrani
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  4. Syed Waqif Husain
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Correspondence to Syed Waqif Husain.

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Mashayekhi, H.A., Abroomand-Azar, P., Saber-Tehrani, M. et al. Rapid Determination of Carbamazepine in Human Urine, Plasma Samples and Water Using DLLME followed by RP–LC. Chroma 71, 517–521 (2010). https://doi.org/10.1365/s10337-009-1456-6

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  • DOI: https://doi.org/10.1365/s10337-009-1456-6

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