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Simultaneous Separation of Four Flavonoids and Two Astragalosides from Radix Astragali by Semi-Preparative LC

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Abstract

An effective method for simultaneous separation of four flavonoids and two astragalosides was developed by semi-preparative high-performance liquid chromatography in this work. A crude sample was firstly cleaned-up from ethanol aqueous extract of Radix Astragali by LS-300B resin-based column chromatography and was further isolated by semi-preparative LC with a gradient elution of water–methanol. Six compounds including calycosin (3.5 mg), formononetin (2.6 mg), (6aR,11aR)-10-hydroxy-3,9-dimethoxypterocarpan (2.6 mg), (3R)-7,2′-dihydroxy-3′,4′-dimethoxyisoflavan) (2.0 mg), astragaloside I (5.4 mg) and astragaloside II (9.8 mg) were obtained with a recovery of 67.8, 77.2, 88.4, 85.3, 62.1 and 57.9%, respectively. Their chemical structures were confirmed by MS and NMR analysis. This study developed an effective and rapid method for simultaneous separation of multiple components from Radix astragali.

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Acknowledgment

This work was supported by the International Co-operative Project of Science and Technology from the Ministry of Science and Technology of People’s Republic of China (contract/grant number: 2007DFA40680) and National Nature Foundation Committee of P.R. China (Grants No. 20875104). The authors would like to thank Lu Huang and Li Hu (Hunan institute of chemical industry, Changsha, China) for the NMR and the MS analysis.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Research Center of Modernization of Chinese Medicines, Central South University, 410083, Changsha, People’s Republic of China

    Xinrong Dong, Yizeng Liang & Bing Wang

  2. College of Science, Hunan Agricultural University, 410128, Changsha, People’s Republic of China

    Xinrong Dong & Xiaohong Long

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  1. Xinrong Dong
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  2. Yizeng Liang
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Correspondence to Yizeng Liang.

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Dong, X., Liang, Y., Wang, B. et al. Simultaneous Separation of Four Flavonoids and Two Astragalosides from Radix Astragali by Semi-Preparative LC. Chroma 71, 225–231 (2010). https://doi.org/10.1365/s10337-009-1437-9

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