Abstract
Chinese clematis is a Chinese medicine with anti-inflammatory and anti-rheumatoid properties. Studies showed the triterpenoid saponins of this herb represent the major active components with therapeutic activities for the treatment of inflammatory diseases. The LC–ELSD method was developed to quantitatively analyze seven major triterpenoid saponins in 22 samples from different habitats. The linearity, sensitivity, precision, and accuracy were investigated. It showed the great variation of the contents of each saponin. We concluded clematichinenoside AR is the major saponin in all the samples. This method can be used to effectively evaluate and monitor the quality of Chinese clematis in clinical use.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
The State Pharmacopoeia Commission of The People’s Republic of China (2005) Pharmacopoeia of the People’s Republic of China. Chem Ind Press Beijing 1:175
Li S (1979) Compendium of material medical. People’s Public Health Press, Beijing, pp 1308–1309
Lv D (1999) Strait Pharm J 11(4):7–9
Xu X, Xia L, Dai M, Peng D (2005) Pharmacol Clin Chin Materia Med 21(4):34–35
Qiu G, Zhang M, Yang Y (1999) Zhong Yao Cai 22(7):351–353
Mimaki Y, Yokosuka A, Hamanaka M, Sakuma C, Yamori T, Sashida Y (2004) J Nat Prod 67(9):1511–1516. doi:10.1021/np040088k
Shao B, Qin G, Xu R, Wu H, Ma K (1996) Phytochemistry 42(3):821–825. doi:10.1016/0031-9422(95)00875-6
Xu R, Zhao W, Xu J, Shao B, Qin G (1996) Adv Exp Med Biol 404:371–382
Shao B, Qin G, Xu R, Wu H, Ma K (1995) Phytochemistry 38(6):1473–1479. doi:10.1016/0031-9422(94)00899-5
Shi S, Jiang D, Zhao M, Tu P (2007) J Chromatogr B Analyt Technol Biomed Life Sci 852:679–683. doi:10.1016/j.jchromb.2007.02.056
Shi S, Jiang D, Dong C, Tu P (2006) J Nat Prod 69(11):1591–1595. doi:10.1021/np060287z
Kawata Y, Kizu H, Miyaichi Y, Tomimori T (2001) Chem Pharm Bull (Tokyo) 49(5):635–638. doi:10.1248/cpb.49.635
Kawata Y, Kizu H, Tomimori T (1998) Chem Pharm Bull (Tokyo) 46(12):1891–1900
Li FW, Liu LF, Li YM, Wang YX, Zhu XX (2005) Chinese Patent, ZL 2005100408242, authorized in Aug 2007
Huang W, Yang H, Wang Q, Yuan C, Xu X (2004) Nat Prod Res Dev 16(4):338–339
Xu T, Li C, Wan D (2005) Res Inf Tradit Chin Med 7(8):121–122
Xu T, Wan D (2005) Zhongguo Zhong Yao Za Zhi 30(17):1383–1384
Xiong H, Zhen M (2007) Zhongguo Zhong Yao Za Zhi 32(4):357–359
Yang L, Pei J, Wan D (2006) Nat Prod Res Dev 18:313–315
Sun F, He Q, Xiao P et al (2008) Phytochem Anal 19:40–45. doi:10.1002/pca.1013
Kizu H, Shimana H, Tomimori T (1995) Chem Pharm Bull (Tokyo) 43(12):2187–2194
Acknowledgments
Financial supports from the National Natural Science Foundation of China (Project No. 30772770), High Technology Foundation Program of Jiangsu Province (No. BG2007613) and National Undergraduate Students’ Innovative Experimental Program of Ministry of Education of People’s Republic of China are gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ma, X., Xie, L., Liu, L. et al. Simultaneous Quantification of Seven Main Triterpenoid Saponins in Radix et Rhizoma Clematidis by LC–ELSD. Chroma 69, 437–443 (2009). https://doi.org/10.1365/s10337-008-0906-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-008-0906-x