Abstract
For the first time a simple, rapid, and specific liquid chromatographic–mass spectrometric (LC–MS) method for simultaneous quantification of 15 lignans from Schisandra chinensis (Turcz.) Baill. fruit has been developed and fully validated. Samples were ultrasonically extracted with methanol. Chromatographic separation was performed on a C18 column with methanol–0.1% aqueous acetic acid 72:28 (v/v) as mobile phase at a flow rate of 0.8 mL min−1; the run time was 40 min. The analytes were monitored by selected ion monitoring (SIM) with electrospray ionization (ESI). Fifteen regression equations revealed good linear relationships (r 2 > 0.99) between peak area and concentration. Within-batch and between-batch precision of the method for the 15 lignans was <9.5% and accuracy was 93.1–107.5%. The validated method was successfully used to determine the 15 compounds in the fruits of Schisandra chinensis procured from different regions of China. The results indicated the method could be used for quality control of Schisandra chinensis fruit and might also useful for further studies of lignans.
Similar content being viewed by others
References
Takeda S, Arai I, Hasegawa M, Tatsugi A, Aburada M, Hosoya E (1988) Nippon Yakurigaku Zasshi 91:237–244
Hancke JL, Burgos RA, Ahumada F (1999) Fitoterapia 70:451–471
Lu H, Liu GT (1992) Planta Med 58:311–313
Chiu PY, Mak DH, Poon MK, Ko KM (2002) Planta Med 68:951–956
Kim SR, Lee MK, Koo KA, Kim SH, Sung SH, Lee NG, Markelonis GJ, Oh TH, Yang JH, Kim YC (2004) J Neurosci Res 76:397–405
Seo SM, Lee HJ, Park Y, Lee MK, Park JI, Paik KH (2004) Nat Prod Sci 10:104–108
Zhu XH, Shen Q, Gao TM (2004) Chin Tradit Herbal Drugs 35:122–124
Cheng JY (2007) Cap Med 7:50–51
Opletal L, Sovova H, Bartlova M (2004) J Chromatogr B 812:357–371
Chen Y, Qin G, Xie Y (2001) Zhong Yao Cai 24:105–107
Kvasnickova L, Glatz Z, Sterbova H, Kahle V, Slanina J, Musil P (2001) J Chromatogr A 916:265–271
Bartlova M, Opletal L, Chobot V, Sovova H (2002) J Chromatogr B 770:283–289
Sterbova H, Sevcikova P, Kvasnickova L, Glatz Z, Slanina J (2002) Electrophoresis 23:253–258
Wang YH, Gao JP, Chen DF (2003) Zhongguo Zhong Yao Za Zhi 28:1155–1160
Chen A, Li C, Gao W, Hu Z, Chen X (2005) Biomed Chromatogr 19:481–487
Tian K, Qi S, Cheng Y, Chen X, Hu Z (2005) J Chromatogr A 1078:181–187
Avula B, Choi YW, Srinivas PV, Khan IA (2005) Chromatographia 61:515–518
He X, Lian L, Lin L (1997) J Chromatogr A 757:81–87
Huang X, Song F, Liu Z, Liu S (2007) J Mass Spectrom 42:1148–1161
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) Chem Pharm Bull 27:1576–1582
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) Chem Pharm Bull 27:2695–2709
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1978) Chem Pharm Bull 26:3257–3260
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) Chem Pharm Bull 27:1583–1588
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) Chem Pharm Bull 27:1383–1394
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) Chem Pharm Bull 27:1395–1401
Ikeya Y, Taguchi H, Yosioka I (1980) Chem Pharm Bull 28:2422–2427
Ikeya Y, Taguchi H, Yosioka I (1981) Chem Pharm Bull 29:2893–2898
Ikeya Y, Taguchi H, Yosioka I (1982) Chem Pharm Bull 30:132–139
Ikeya Y, Ookawa N, Yosioka I, Kobayashi H (1982) Chem Pharm Bull 30:3202–3206
Nakajima K, Taguchi H, Ikeya Y, Endo T, Yosioka I (1983) Yakugaku Zasshi 103:743–749
Ikeya Y, Kanatani H, Hakozaki M, Taguchi H, Mitsuhashi H (1988) Chem Pharm Bull 36:3974–3979
Ikeya Y, Taguchi H, Mitsuhashi H, Takeda S, Kase Y, Aburada M (1988) Phytochemistry 27:569–573
Zhai HB, Cong PZ (1990) Yao Xue Xue Bao 25:110–122
Ikeya Y, Taguchi H, Yosioka I, Kobayashi H (1979) Chem Pharm Bull 27:1576–1582
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Deng, X., Chen, X., Cheng, W. et al. Simultaneous LC–MS Quantification of 15 Lignans in Schisandra chinensis (Turcz.) Baill. Fruit. Chroma 67, 559–566 (2008). https://doi.org/10.1365/s10337-008-0589-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-008-0589-3