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Simultaneous Determination of Quercetin and Luteolin in Dried Flowers by Multivariate HPLC-ECD Calibration

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Abstract

Two calibration models, partial least squares (PLS) and principal component regression (PCR), were applied to the simultaneous determination of quercetin and luteolin by high performance liquid chromatography (HPLC) with electrochemical detection (ECD). The proposed methods were successfully applied to the analysis of dried flower samples. It was found that the relative standard errors of prediction (RSEP) for the validation set of PLS of quercetin and luteolin was 1.31 and 2.23%, and the RSEP of PCR was found to be 3.56 and 4.32%, respectively. Several dried flower samples were analysed and the recoveries were in the range of 95.9–103.3%.

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Acknowledgment

The authors gratefully acknowledge the financial support of this project by the National Science Foundation of China (No. 20675062) and the Natural Science Foundation of Shaanxi Province of China (No.2004B20).

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Authors and Affiliations

  1. Institute of Analytical Science, Shaanxi Province Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi′an, 710069, China

    Yuan-Zhen Zhou, Xiao-Xing Liu, Xiao-Hui Zheng & Jian-Bin Zheng

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  1. Yuan-Zhen Zhou
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  2. Xiao-Xing Liu
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  3. Xiao-Hui Zheng
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  4. Jian-Bin Zheng
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Correspondence to Jian-Bin Zheng.

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Zhou, YZ., Liu, XX., Zheng, XH. et al. Simultaneous Determination of Quercetin and Luteolin in Dried Flowers by Multivariate HPLC-ECD Calibration. Chroma 66, 635–637 (2007). https://doi.org/10.1365/s10337-007-0363-y

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  • DOI: https://doi.org/10.1365/s10337-007-0363-y

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